用高效液相色谱法同时测定3种抗癫痫药血药浓度  

HPLC method for simultaneous determination of three antiepileptic concentrations in serum

作　　者：张金泉[1] 杨志英[1] 任家佩[1] 蔡佳 

机构地区：[1]中日友好医院药剂科,100029 [2]天津第二医学院药学系

出　　处：《中国药学杂志》1992年第1期19-20,共2页Chinese Pharmaceutical Journal

摘　　要：本文采用高效液相色谱法[HPLC]同时测定笨巴比妥、苯妥英和卡马西平3种抗癫痫药物,用环巴比妥[5-(1-cyclohexen-1-yl)-5-ethyl-barbituric acid]作内标物。以乙腈沉淀样品中血清蛋白后,于 ODS 色谱柱上分离,检测波长为254nm。3种药物分离良好,本法简便易操作,干扰少,适用于临床病人抗癫痫药物血浓度监测。A method was developed for simultaneous de-termination of phenobarbital,phenytoin and car- bamzepine in serum by HPLC.The procedure in-volved precipitation serum protein with acetonitrile(CH_3CN),directive in;ection in a reverse-phaseliquid chromatography column,cyclobarbital 5-(1-cyclohexen-1-yl)-5-ethyl-barbituric acid wasused as internal standard and the detector wave-length was 254nm.Three drugs were separatedwith this simple method.The method can be usedin monitoring therapeutic drugs used in antiepilep-tic patients.

关 键 词：苯巴比妥 抗癫痫药 血药浓度 HPLC 

分 类 号：R971.6[医药卫生—药品]