HPLC测定十二味齿龈康散中大黄素、大黄酚的含量  

Determination of emodin and chrysophnol in Shierwei Chiyinkang Powder by HPLC

作　　者：刘晖[1] 段东波[1] 常俊 曹朝霞 

机构地区：[1]兰州医学院,甘肃兰州730000 [2]甘肃省药物研究所,甘肃兰州730020

出　　处：《中成药》2004年第4期292-294,共3页Chinese Traditional Patent Medicine

摘　　要：目的 :同时测定十二味齿龈康散 (寒水石 ,大黄 ,细辛 ,川芎 ,羌活 ,龙胆草 ,花椒等 )中有效成分大黄素、大黄酚的含量。方法 :参照《中国药典》2 0 0 0版一部制备样品溶液 ,经高效液相色谱仪测定。Shim packCIC ODS柱 ( 15 0mm× 5mm ,4 .5 μm) ,流动相为甲醇 0 .1%磷酸 ( 4 0∶9) ,流速 1.3mL·min-1,检测波长 2 5 4nm。结果 :样品中大黄素、大黄酚与其它成分有良好分离。大黄素的线性范围为 0 .5 2～ 5 .2 8μg·mL-1,r =0 .9999,平均回收率为 98.6 2 % ;大黄酚的线性范围为 0 .96～ 9.6 0 μg·mL-1,r =0 .9999,平均回收率为 98.5 7%。结论 :本法灵敏度高 ,结果准确 ,可作为十二味齿龈康散的含量测定方法。AIM: To develop a HPLC method to determine emodin and chrysophanol in Shierwei Chiyinkang Powder (Gypsumseu Calcitum, Radix et Rhizoma Rhei, Herba Asari, Rhizoma Chuanxiong, Rhizoma et Radix Notopterygii, Radix Gentianae, Pericarpium Zanthoxyli, etc.). METHODS: The sample was made referring to China Pharmacopoeia(2000) and determined by HPLC. The separation was performed on a Shim pack CIC ODS column (150mm×5mm, 4.5μm) with a mobile phase of methanol 0.1% H 3PO 4 solution (40∶9). The flow rate was 1.3mL·min -1 and the detective wavelength was set at 254nm. RESULTS: The emodin and chrysophanol in sample were separated. The square linear ranges were 0.52～5.28μm·mL -1 ( r =0.9999) and 0.96～9.60μm·mL -1 ( r=0.9999 ), respectively, and the average recoveries were 98.62% and 98.57% for emodin and chrysophanol, respectively. CONCLUSION: This method is sensitive and accurate for the determination of emodin and chrysophanol in the sample.

关 键 词：十二味齿龈康 大黄素 大黄酚 HPLC 

分 类 号：R927.2[医药卫生—药学]