HPLC测定不同产地丹参药材中脂溶性成分  

Determination of fat-soluble component in Radix Salviae miltiorrhizae from different sources

作　　者：安睿[1] 王新宏[1] 周思丽[1] 尤丽莎[1] 王智华[1] 洪筱坤[1] 王依群 

机构地区：[1]上海中医药大学化学教研室,上海201203 [2]上海华宇药业有限公司,上海200002

出　　处：《中成药》2004年第4期294-297,共4页Chinese Traditional Patent Medicine

摘　　要：目的 :研究不同产地丹参中二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA 含量和测定方法。方法 :用HPLC法测定 ,以 1%醋酸甲醇溶液和 1%醋酸水溶液为流动相作梯度洗脱 ,检测波长为 2 80nm。结果 :所测 4种成分的平均回收率均大于 97% ,RSD小于 1.2 % ,线性良好。结论 :本分析方法稳定可靠 ,重现性好。AIM: To study the contents and determination method of dihydrotanshinone I, cryptotanshinone, tanshinone I, tanshinone IIA in Danshen from different sources. METHODS: Determination by HPLC with the gradient eluent consisted of methanol (1% acetic acid) water (1% acetic acid). The detection wavelength was at 280 nm. RESULTS: The average recovery of four components were all above 97%, RSD was no more than 1.2%. The calibration curve was linear. CONCLUSION: The method is accurate and reliable with good reproducibility.

关 键 词：丹参 二氧丹参酮Ⅰ 隐丹参酮 丹参酮Ⅰ 丹参酮ⅡA HPLC 

分 类 号：R284.1[医药卫生—中药学]