人表皮生长因子的三种分离方法及其生物学活性  

THREE ISOLATION METHODS OF HUMAN EPIDERMAL GROWTH FACTOR AND ITS BIOLOGICAL ACTIVITY

作　　者：李守芬 苏钟浦[1] 王立坤[1] 刘玉艳[1] 王彦[2] 陈子易[3] 

机构地区：[1]中国医科大学基础医学部免疫化学研究室 [2]中国医科大学基础医学部组胚教研室 [3]中国医科大学基础医学部化学检测中心

出　　处：《中国医科大学学报》1992年第3期172-174,共3页Journal of China Medical University

摘　　要：人表皮生长因子(hEGF)是细胞生长因子之一。本文提出了从人尿中分离hEGF的简单、易行的新方法,并与另外二种方法做了比较。其生物活性用角膜组织培养后,能使角膜上皮,前界膜明显增厚,并使角膜固有层的成纤维细胞的数目明显增多。Human epidermal growth factor (hECF) is one of cell growth factors. hEGF was isolated by three isolation methods including a new one from human urine.hEGF was investigated with rabbit corneal epithelial cell(CEC). CEC were cultured by tissue block technique and observed with upsetting microscope and tissue slice technique. The results showed hEGF accelerated the corneal epithelium growths, especially, substantia propria corneae.

关 键 词：角膜上皮 分离 人表皮生长因子 

分 类 号：R392－33[医药卫生—免疫学]