尖锐湿疣患者疣体HPV多型别原位杂交检测  

Detection of Multiple Type Human Papillomavirus in Condyloma Acuminata Tissues by in Situ Hybridization Technique

作　　者：吴文中[1] 佟菊贞[2] 林汉良[3] 吴惠茜[3] 张书岭[1] 

机构地区：[1]深圳市第二人民医院皮肤科,广东深圳518035 [2]中山大学第一医院皮肤科,广东广州510080 [3]中山大学病理教研室,广东广州510089

出　　处：《中国皮肤性病学杂志》2004年第4期231-232,共2页The Chinese Journal of Dermatovenereology

摘　　要：目的　探讨原位分子杂交技术多型别检测尖锐湿疣患者疣体HPV DNA的敏感性。方法　应用原位杂交法 ,通过广谱生物素标记的HPV DNA探针 ,对尖锐湿疣患者疣体HPV 6,11,16,18,3 0 ,3 1,3 3 ,3 5 ,45 ,5 1,5 2型DNA进行检测。结果　在 3 5例标本中 ,有 3 3例HPV DNA呈阳性反应 ,阳性率 94.3 %。阳性反应物主要分布于表皮浅层空泡细胞的细胞核内 ,多呈片状或灶状分布。结论　用广谱生物素标记的原位杂交技术 ,可检测HPV多型别 ,具有敏感性高、特异强的优点 。Objective To explore the sensitivity of detection of human papillomavirus in condyloma acuminata tissues by in situ hybridization technique.Methods In situ hybridization(ISH) technique was used to detect HPV-DNA (HPV 6,11,16,18,30,31,33,35,45,51,52) in condyloma acuminata tissues by HPV broad-spectrum probes.Results There were 33 positive cases in 35 patients with condyloma acuminata,94.3% of condyloma acuminata tissues were positive for HPV-DNA with ISH.The major positive reactant located in the nucleus of vacuolar cells in superficial epidermsis,and distributed flakily or focally.Conclusion In situ hybridization with broad-spectrum probes can detect multipul type HPV-DNA sensitively and specially,and locate position of the virus in histopathology of condyloma acuminata.

关 键 词：尖锐湿疣 HPV 原位分子杂交 检测 人乳头瘤病毒 

分 类 号：R752.53[医药卫生—皮肤病学与性病学] R446[医药卫生—临床医学]