传染性软疣病毒MC148基因的克隆和序列分析  

Cloning and analysis of MC148 gene of molluscum contangiosum virus

作　　者：孙星慧[1] 谭建明[1] 严伟[1] 吴卫真[1] 周浩[1] 林文洪[1] 

机构地区：[1]南京军区福州总医院泌尿外科,福建福州350025

出　　处：《医学研究生学报》2004年第4期300-301,314,共3页Journal of Medical Postgraduates

摘　　要：目的 :克隆传染性软疣病毒 (MCV)的MC14 8基因 ,研究其基因序列。　方法 :从传染性软疣患者皮肤上的软疣小体中提取病毒DNA ,经PCR扩增并测序后构建于 pUC19克隆载体上。对重组体pUC19 MC14 8再次进行测序。　结果 :扩增出约 315bp的片段 ,PCR产物及克隆产物测序证明 ,基因序列与GenBank报道的相同。　结论 :成功克隆出MCV的MC14 8基因 ,为趋化因子拮抗剂的研究奠定基础。Objective:To clone MC148 gene of molluscum contagiosum virus(MCV). Methods:MCV DNA was extracted from the specimens of patients with multiple molluscum lesions and amplified. The product was then cloned into vector pUC19 and sequenced . Result:The sequence of MC148 gene with 315bp cloned was identical to that reported in GenBank. Conclusion:The MCV MC148 gene was cloned successfully.

关 键 词：趋化因子 传染性软疣病毒 基因克隆 聚合酶链反应 

分 类 号：R392.12[医药卫生—免疫学]