整合素表达对伤口愈合时成纤维细胞合成原胶原的影响  

Effect of integrin expression on procollagen synthesis of fibroblasts in wound healing

作　　者：陈敏亮[1] 柴家科[1] 林子豪[2] 宋慧锋[1] 

机构地区：[1]解放军第304医院烧伤整形外科,北京100037 [2]第二军医大学长征医院整形外科

出　　处：《中华医学美学美容杂志》2003年第3期166-168,共3页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：国家自然科学基金 (3 9670 73 );中国博士后科学基金资助项目

摘　　要：目的　研究伤口愈合时创面成纤维细胞整合素表达对成纤维细胞合成原胶原的影响。方法　以反义寡核苷酸阻断创面成纤维细胞表面相应整合素亚基的表达 ,用反转录 多聚酶链式反应RT PCR检测该整合素表达被抑制后创面成纤维细胞原胶原mRNA量的变化。结果　反义寡核苷酸能特异性抑制创面成纤维细胞相应整合素亚基的表达 ,该成纤维细胞合成原胶原α1(Ⅰ )、α1(Ⅲ )mR NA的量显著降低。结论　降低创面成纤维细胞整合素的表达可从转录水平抑制成纤维细胞合成原胶原 ,为伤口愈合时减少瘢痕产生的研究提供实验依据。Objective To investigate the effect of integrin expression on procollagen synthesis of fibroblasts in wound healing. Methods Antisense oligonucleotides were used to intercept the expression of integrin α 5 or β 1 subunit on fibroblasts respectively in wound healing, then the changes of procollagen mRNA were detected by RT-PCR. Results The antisense oligoncleotdes could specifically inhibit the expression of integrin subunit. Decreased expression of integrinα5 or β1 subunit on fibroblasts significantly lowered mRNA of procollagen α 1(Ⅰ) and α 1(Ⅲ). Conclusion Reduction of integrin expression on fibroblasts in wound may inhibit procollagen synthesis in the level of transcription. These results provide the experimental gists on studying of scar reduction in wound healing.

关 键 词：整合素表达 伤口愈合 成纤维细胞 原胶原 ECM 临床资料 

分 类 号：R641[医药卫生—外科学]