褪黑素对T淋巴细胞株免疫相关基因表达谱的作用  

Effect of melatonin on immuno-related gene expressi on profile of Tlymphocyte line

作　　者：陈向芳[1] 刘志民[1] 赵瑛[2] 邓安梅[3] 林炜栋[4] 阮晔[1] 彭树勋[5] 

机构地区：[1]上海第二军医大学附属长征医院内分泌科,200003 [2]上海第二军医大学附属长征医院神经内科,200003 [3]上海第二军医大学附属长征医院实验诊断科,200003 [4]上海第二医科大学附属瑞金医院烧伤研究所 [5]香港大学医学院生理系

出　　处：《中华内分泌代谢杂志》2003年第5期395-398,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：香港裘槎基金资助项目 ( 396 6 30 0 8)

摘　　要：目的　利用基因表达谱芯片研究T淋巴细胞株 (HUT78)在褪黑素作用后免疫相关基因表达的差异性。方法　用Cy5和Cy3两种不同的荧光染料通过逆转录反应将干预组和对照组的HUT78细胞的mRNA分别标记成两种探针 ,并与载有一组靶基因的基因表达谱芯片进行杂交 ,通过扫描荧光强度 ,计算机软件分析 ,寻找经褪黑素作用后表达有差异的基因。结果　T淋巴细胞株受褪黑素作用 2 4h后 ,共筛选出 42条差异表达基因 ,其中 3 5条基因上调 ,7条基因下调。结论　褪黑素激活的人淋巴细胞 。Objective To in ve stigate the difference of immuno-related gene expressions in T lymphocyte line (HUT78) incubated with melatonin by cDNA microarray of gene expression profile. Methods Two cDNA probes labelled with Cy3 or C y5 fluorescence dyes were prepared from mRNA of melatonin-treated or untreated HUT78 cells and were hybridized with cDNA microarray of target gene expression p rofile which were scanned for fluorescent intensity. The difference of gene expr ession profile was screened by computer software. Results After T lymphocyte line was incubated with melatonin for 24 h, 42 ge nes showed different expressions, among them 35 of immuno-related genes were up regulated and 7 were downregulated. Conclusion Human lymphocytes activated by melatonin play the immuno-regulatory roles throu gh regulation of a number of gene expressions.

关 键 词：褪黑素 T淋巴细胞株 免疫相关基因 表达谱 

分 类 号：R392[医药卫生—免疫学]