用脉冲伏安法观察多巴胺D_1D_2受体拮抗剂对大鼠纹体中DOPAC的影响  

The Effects of Dopamine D-1 and D-2 Receptor Antagonists on 3,4-dihydroxyphenylacetic acid (DOPAC) in Rat Striatum with Differential Normal Pulse Voltammetry

作　　者：汪晓芬 钮心懿[1] 

机构地区：[1]中国医学科学院药物研究所

出　　处：《中国医学科学院学报》1992年第4期257-262,共6页Acta Academiae Medicinae Sinicae

摘　　要：本文用脉冲伏安法在体测定麻醉大鼠纹体中二羟苯乙酸(DOPAC)的含量,并观察多巴胺受体拮抗剂氟哌啶醇(D_2/D_1)、氯丙嗪(D_2/D_1),舒必利(D_2)以及SCH23390(D_1)对纹体中DOPAC的影响。结果表明这些多巴胺受体拮抗剂均可使麻醉大鼠纹体中DOPAC含量增加,与基础DOPAC浓度(14±2μmol/L)相比,氟哌啶醇(0.5mg/kg s.c.),氯丙嗪(5mg/kgs.c.)、舒必利(5mg/kg s.c),SCH23390(1mg/kg s.c.)分别使DOPAC增加140％、102％、40％以及26％。从而证实不仅突触前D_2受体参与反馈调节DA的合成代谢,而且D_1受体也参与以上反馈调节机制,但D_2受体的调节作用较强。Dopamine metabolite 3, 4-dihydroxyphenylacetic acid (DOPAC) was detected by differential normal pulse voltammetry (DNPV) in chloral hydrate anesthetized rat striatum. The effects of some dopamine D-1 and D-2 antagonists on DOPAC concentration were observed in order to demonstrate the role of presynaptic D-1 and D-2 receptors in the feedback of dopamine synthesis arid metabolis.n. The results showed that the mixed antagonists haloperidol (0.5mg/kg s.c.) and chlorpromazine (5 mg/kg s.c.), the selective D-l antagonist SCH23390 (1 mg/kg s.c.), and the selective D-2 antagonist sul-piride(5 mg/kg s.c.) all increased DOPAC concentration significantly. The percentages of increase were 140%, 102%, 26% and 40% in comparison with basal DOPAC concentration (14×2 μmol/L), respectively, These results indicate that not only presynaptic D-2 receptor regulates DA synthesis and metabolism, but that the presynatic D-1 receptor also takes part in this feedback mechanism.

关 键 词：脉冲伏安法 多巴胺 受体拮抗剂 

分 类 号：R966[医药卫生—药理学]