克林霉素磷酸酯栓中克林霉素磷酸酯的含量测定  

Determination of clindamycin phosphate for clindamycin phosphate suppositories by HPLC

作　　者：胡容峰[1] 彭代银[1] 戈文兰[2] 吴德林[1] 

机构地区：[1]安徽中医学院药学系,安徽合肥230038 [2]江苏省南京药物研究所,江苏南京210009

出　　处：《中国药学杂志》2004年第3期222-223,共2页Chinese Pharmaceutical Journal

摘　　要：目的　采用高效液相色谱法测定克林霉素磷酸酯栓中克林霉素磷酸酯的含量。方法　用SymmetryShieldC18柱 (5 μm ,2 5 0mm× 4 6mm) ,以乙腈 枸橼酸溶液 (取枸橼酸 1 0 5g ,加水溶成 10 0 0mL ,即得 ) (2 2∶78)为流动相 ,用紫外检测器于 2 10nm处检测。结果　线性范围为 99 36～ 14 9 0 4 μg·mL-1(r=0 9997)。平均回收率为 10 1 2 % ,RSD =0 2 5 % (n =3)。结论　本法操作简便 ,分析准确快速 ,干扰小。OBJECTIVE: A HPLC method was established for the determination of clindamycin phophate for clindamycin phophate suppositories. METHODS: Clindamycin phophate was separated on Symmetry Shield C18 column (5 μm, 250 mm × 4.6 mm) with citric acid solution (citric acid 1.05 g dissolved in water and diluted to 1000 mL)-acetonitrile (78:22) as the mobile phase and detected at 210 nm. The flow rate was 1.0 mL&middotmin-1. RESULTS: The standard curve was linear over the concentration range of 99.36 - 149.04 μg&middotmL-1 and the correlation coefficient was 0.9997. The average recovery and the relative standard deviation was 101.2%, and 0.25% respectively. CONCLUSION: The method is simple, rapid, accurate and reliable.

关 键 词：克林霉素磷酸酯栓 克林霉素磷酸酯 含量测定 高效液相色谱法 

分 类 号：R978.1[医药卫生—药品] R927.2[医药卫生—药学]