应用液相色谱法分离纯化固相化学合成胸腺素α_1  

APPLICATION OF LIQUID CHROMATOGRAPHY FOR ISOLATION AND PURIFICATION OF SOLID-PHASE SYNTHETIC THYMOSIN α_1

作　　者：甘一如[1] 黄永东[1] 吴蕾[1] 

机构地区：[1]天津大学化工学院,天津300072

出　　处：《磁流体发电情报》2004年第3期497-500,共4页

基　　金：天津市自然科学基金资助项目 (No 993 80 43 11)

摘　　要：By using ion-exchange preparative chromatography (IEPC) and reversed-phase high performance liquid preparative chromatography(RP-HPLPC), thymosin α 1 was isolated and purified from the crude product synthesized by the solid-phase peptide synthesis(SPPS) method. The purity of the final product reached 95% through ion-exchange chromatography on DEAE Sepharose Fast Flow chromatography and Delta-Pak TM C18 purification after optimizing the chromatographic conditions. The capacity of purification process was 50mg/circle. The total yield was 36%. The technology is simple and reliable, and can be scaled up easily.By using ion-exchange preparative chromatography (IEPC) and reversed-phase high performance liquid preparative chromatography(RP-HPLPC), thymosin α 1 was isolated and purified from the crude product synthesized by the solid-phase peptide synthesis(SPPS) method. The purity of the final product reached 95% through ion-exchange chromatography on DEAE Sepharose Fast Flow chromatography and Delta-Pak TM C18 purification after optimizing the chromatographic conditions. The capacity of purification process w...

关 键 词：胸腺素Α1 液相色谱法 分离 纯化 固相化学合成 

分 类 号：TQ460.6[化学工程—制药化工]