家蚕卵温汤处理酯酶A4的活性变化  

Changes of Esterase a4-activity in Hot Water Treated Eggs of Silkworm, Bombyx mori

作　　者：徐世清[1,2] 司马杨虎[1,2] 陈息林[1] 戴璇颖[1] 

机构地区：[1]苏州大学农业科学与技术学院 [2]苏州大学蚕桑研究所,苏州215006

出　　处：《蚕业科学》2004年第1期50-54,共5页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金 (编号 3 0 3 710 86) ;江苏省留学回国基金 (编号M1113 0 3 0 ) ;江苏省科技厅国际合作项目 (编号LZ113 0 2 1)

摘　　要：温汤孵化法是一种重要的家蚕卵人工孵化方法。酯酶A4 (EsteraseA4 ,EA4 )是家蚕卵的滞育生物钟蛋白质。将家蚕C10 8品种卵温汤处理 ,纯化出EA4 ,测定了EA4的ATP酶 (ATPase)活性变化。结果显示 :产卵后 15h(2 5℃ )的温汤处理蚕卵 ,其EA4的ATP酶最大活性 ,体外 5℃或 2 5℃条件下 ,分别出现在处理后 2 4h或 1.5h ,蚕卵也随即解除滞育 ,获得孵化能力 ;产卵后 30h(2 5℃ )的温汤处理蚕卵 ,其点青卵率随温汤刺激量增大而提高 ,点青时间随刺激量增大而缩短 ;蚕卵EA4的ATPase最大活性出现时间也随温汤刺激量增大而提前 ;且温汤处理蚕卵EA4的活性变化与蚕卵活化有高度相关性。The hot water treatment is an important method for artificial hatching in silkworm eggs. Esterase A4 (EA4), a time-interval measuring enzyme in the diapausing eggs of Bombyx mori, was purified from hot water-treated eggs of Bombyx C108 strain, and has been studied using ATP as a substrate. The ATPase activity peak for EA4 purified from 15 hours old eggs appeared at hour 24 at 5 ℃ or hour 1.5 at 25 ℃ after the treatment, and the diapause of eggs was terminated rapidly. ATPase activity peak for EA4 from 30 hours old eggs is appeared at an early time, the period of incubation shortens and the rate of head pigmentation of embryos enhances, by increasing the hot water stimulus (soaking duration). The changes of ATPase activity for EA4 in hot water treated eggs are highly correlated with eggs activation.

关 键 词：家蚕 卵 温汤处理 酯酶A4 活性 人工孵化 滞育 

分 类 号：S881.2[农业科学—特种经济动物饲养]