家蚕催青期胚胎蛋白质图谱的建立  

Establishment of 2D-PAGE Protein Patterns of Silkworm Embryo During Incubating Stage

作　　者：颜新培[1] 钟伯雄[1] 曹家树[2] 徐孟奎[1] 沈飞英[1] 姚国华[1] 

机构地区：[1]浙江大学动物科学学院蚕蜂分子生物学实验室,杭州310029 [2]浙江大学农业与生物技术学院细胞与分子生物学实验室

出　　处：《蚕业科学》2004年第1期28-33,共6页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金 (编号 3 0 2 710 0 4)

摘　　要：胚胎发育一直是生命科学研究的主题之一。通过家蚕各个时期胚胎蛋白质的研究 ,可以从蛋白质水平探讨胚胎蛋白质的构成和基因顺序表达的规律。应用蛋白质组的研究方法 ,以连续发育的家蚕胚胎为材料 ,采用蛋白质双向电泳技术 ,对催青期各个时期的胚胎蛋白质进行了分离 ,建立了家蚕催青期各个胚胎蛋白质参考图谱。从催青期各个时期的胚胎蛋白质图谱中发现 :从临界期胚胎到气管显现期的胚胎 ,结构基因表达变化不大 ;从点青期开始 ,一些酸性蛋白有关的结构基因被激活表达。Embryo development is always one of the most important subjects in life sciences. The composing of embryo proteins and rule of gene sequential expression at protein level can be discussed by researching the proteins of every embryo development stage of silkworm. The proteins of every embryo development stage were separated and the protein patterns were established employing continuous developmental embryo during incubating stage of silkworm using two-dimensional electrophoresis. The results indicated that there was little difference in structural gene expression from longest stage embryo to teachea colouring stage embryo of silkworm, however, after head pigmentation stage embryo of silkworm, some structural genes of acidic proteins were activated.

关 键 词：家蚕 催青期 胚胎 蛋白质图谱 发育 结构基因 

分 类 号：S881.2[农业科学—特种经济动物饲养]