果蝇Drosomycin基因(drs)的克隆鉴定  

Cloning Identification of Drosomycin Gene (drs) of Drosophila melanogaster

作　　者：钟仰进[1] 肖业臣[1] 魏剑波[1] 温硕洋[2] 黄亚东[3] 邓小娟[2] 桑延霞[1] 段云[1] 曹阳[1] 

机构地区：[1]华南农业大学蚕学分子生物学与生物技术实验室,广州510642 [2]华南农业大学昆虫学系基因多样性实验室 [3]暨南大学医药生物技术研究开发中心

出　　处：《蚕业科学》2004年第1期85-89,共5页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金 (编号 3 0 170 14 6) ;广东省自然科学基金(编号 0 10 2 90 ) ;国家教委高等学校骨干教师资助计划项目(编号 2 0 0 0 40 )资助

摘　　要：为了克隆Drosomycin基因用于今后定点诱变修饰和生物技术开发研究 ,根据果蝇Drosomycin基因 (drs)的cDNA序列 ,分别设计Drosomycin基因含和不含信号肽序列的两对特异引物Drs1/Drs2、Dro3/Dro4 ,由黑腹果蝇(DrosophilamelanogasterMeigen)基因组PCR扩增获得目的片段drs和dro ,克隆到表达载体pET 2 1d(+)和pHIL S1之中。经测序表明 ,克隆的drs与文献报道的drs序列仅在 +116位点有 1个碱基的差异 (A/T) ,而克隆的dro序列则与文献报道的dro序列完全相同。To clone Drosomycine gene for modification of point mutagenesis and biotechnique utilization, two pairs of the special primers, Drs1/Drs2 and Dro3/Dro4, with or without signal peptide sequence, respectively, were designed according to cDNA sequence of Drosomycine gene (drs) to obtain target drs and dro fragments by PCR from the genome of Drosophila melanogaster Meigen.The amplified fragments of drs and dro were cloned into expression vector pET-21d(+)and pHIL-S1, respectively. The results of the sequencing drs and dro fragments inserted two vectors showed that there is only one base difference (+116 locus: A/T) between the cloned drs sequence and the drs sequence in the references, the cloned dro sequence is quite same as the dro sequence in the references.

关 键 词：果蝇 Drosomycin基因 昆虫抗真菌肽 信号肽 克隆 

分 类 号：Q785[生物学—分子生物学]