洛伐他汀促进成骨细胞增殖、BMP-2表达和矿化的实验研究  

EXPERIMENTAL STUDY ON LOVASTATIN PROMOTING PROLIFERATION,BMP-2 EXPRESSION AND MINERALIZATION OF OSTEOBLASTS

作　　者：杨允[1] 张佐伦[1] 胡坚莉[2] 孙建民[1] 王大川[1] 王松刚[1] 张立平[2] 

机构地区：[1]山东大学山东省立医院脊柱外科,济南250021 [2]山东大学医学院解剖学教研室,济南250021

出　　处：《中国组织化学与细胞化学杂志》2004年第1期91-96,共6页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的　研究洛伐他汀对体外培养大鼠颅骨成骨细胞生物学功能的影响 ,探讨其促进骨形成的作用机制。方法　洛伐他汀作用于体外培养大鼠颅骨成骨细胞 ,化学染色观察对成骨细胞矿化结节形成的影响 ;用免疫细胞化学单标计数测定成骨细胞增殖率及染色吸光度测定BMP 2的表达的变化 ;BMP 2和BrdU免疫双标染色吸光度测定新生成骨细胞BMP 2的表达情况。结果　实验组成骨细胞矿化结节的数量和面积、细胞增殖率及BMP 2的表达明显高于空白对照组 (P<0 0 5 ) ;实验组新生成骨细胞BMP 2的表达显著高于对照组 (P <0 0 1)。结论　洛伐他汀可促进成骨细胞的增殖、分化、BMP 2的表达和矿化结节的形成 ,从而发挥促进骨形成的作用。Objective To study the effect of lovastatin on the biological function of cultured rat calvarial osteoblasts(RCOBs), and to investigate the mechanism of statins in osteogenesis. Method After Lovastatin treatment, the mean number and area of mineralization nodules formed by RCOBs were measured. BrdU labeling ratio represented the cell proliferation rate. Optic density analysis of BMP-2 immunohistochemical statining was used to determine the BMP-2 abundance in osteoblasts. Result The mean number and area of the mineralization nodes, BMP-2 expression of RCOBs and the proliferation rate of cells were greatly elevated(P<0.05), and in particular, the expression of BMP-2 in neonatal RCOBs was increased significantly(P<0.01) in the experimental group when compared with that in the control group. Conclusion Lovastatin can enhance osteogenesis by increasing the proliferation, differentiation,BMP-2 expression and mineralization of RCOBs.

关 键 词：洛伐他汀 成骨细胞 增殖 BMP-2 表达 矿化 颅骨 骨形态发生蛋白 大鼠 

分 类 号：Q441[生物学—生理学] R336[医药卫生—人体生理学]