白细胞介素-1β对Fos蛋白表达的影响及其在癫痫发作中的作用  

EFFECTS OF INTERLEUKIN-1β ON FOS PROTEIN EXPRESSION AND ITS ROLE IN SEIZURE

作　　者：李平[1] 李正莉[1] 朱长庚[1] 刘庆莹[1] 于珊[1] 魏瑛[1] 

机构地区：[1]华中科技大学同济医学院基础医学院解剖学系,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2003年第3期245-249,共5页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的　探讨白细胞介素 1(IL 1)在癫痫发病中的作用。方法　将实验动物分为四组 :A组 (对照组 )、B组 (IL 1β组 )、C组 (IL 1β +Glu ,阈下剂量组 )、D组 (IL 1受体拮抗剂 +IL 1β +Glu ,阈下剂量组 ) ,分别在各组大鼠侧脑室注射上述试剂 ,然后观察动物行为学表现并用免疫组织化学方法检测大脑皮质及海马Fos蛋白的表达。结果　A组无癫痫发作 ,B组发作程度达Ⅱ～Ⅲ级 ,C组为Ⅳ～Ⅴ级 ,D组为Ⅰ～Ⅱ级。在大脑皮质及海马 ,Fos蛋白在A、D组无明显表达 ,B、C组有明显表达 ,而在齿状回 ,A组仅少量表达 ,B、C、D组表达均明显增强。结论　IL 1β可通过其受体介导而诱导癫痫发作 ,与Glu联合应用可促进癫痫发作 ,免疫因子对神经系统的兴奋性有调节作用。Objective To explore the role of interleukin-1β inseizure. Method Experimental rats were divided into four groups: group A (control group), group B (IL-1β), group C (IL-1βwith a sub-threshold dose of Glu), and group D ( IL-1βreceptor antagonist with IL-1β and Glu). The reagents were injected into the lateral ventricle of rats. Besides behavior observations, the expression of Fos protein was examined by means of immunohistochemistry in the cerebral cortex and hippocampus. Result No seizure occurred in group A, while Ⅱ～Ⅲ grade of seizure was observed in group B, Ⅳ～Ⅴ grade in group C and Ⅰ～Ⅱgrade in group D, respectively. Theexpressionof Fos in the cerebral cortex and hippocampus was higher in groups B and C than in groups A and D. Theexpressionof Fos in the dentate gyrus was low in group A but significantly enhanced in groups B, C and D.Conclusion IL-1βcan induce seizure mediated by its receptors. In combination with glutamate, it can facilitatethe seizure. Theimmunocytokine can regulate the excitability of the nervous system.

关 键 词：白细胞介素-1Β FOS蛋白 表达 癫痫 免疫组织化学 免疫因子 

分 类 号：R338.2[医药卫生—人体生理学] R742.1[医药卫生—基础医学]