Aquaporin-1在大鼠睾丸输出小管的免疫组织化学定位研究  

IMMUNOHISTOCHEMICAL LOCALIZATION OF AQUAPORIN-1 IN THE DUCTULI EFFERENTES OF WISTAR RAT

作　　者：张良[1] 黄铁柱[2] 周新华[1] 张泉[1] 王得志[1] 

机构地区：[1]武汉大学医学院人体解剖学系,武汉430071 [2]郧阳医学院人体解剖学系,十堰442000

出　　处：《中国组织化学与细胞化学杂志》2003年第3期291-294,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的　研究正常大鼠睾丸输出小管上皮细胞上Aquaporin 1(AQP 1)的定位分布以期了解其在水重吸收上的作用机制。方法　对正常Wistar大鼠睾丸输出小管进行常规免疫组织化学方法染色观察。结果　在睾丸输出小管非纤毛上皮细胞的刷状缘及基侧部AQP 1阳性表达强烈 ,核上区的胞内体的质膜上也有阳性表达 ;纤毛上皮细胞的纤毛亦呈阳性反应。结论　Aquaporin 1可能与睾丸输出小管非纤毛上皮细胞水重吸收功能有密切关系。Objective To investigate the cellular expression and localization of Aquaporin-1 (AQP-1) in the efferent ductules of testis.Method Light micrscopy and immunocytochemistry were utilized to localize AQP-1 in the efferent ductules.Result In the efferent ductules, AQP-1 was expressed on the microvilli, basolateral plasma membranes, and apical endosomes of the nonciliated cells and on the cilia of the ciliated cells.Conclusion These results suggest that AQP-1 plays an important role in the fluid reabsorption in the efferent ductules.

关 键 词：AQUAPORIN-1 大鼠 睾丸输出小管 水通道蛋白 免疫细胞化学 

分 类 号：Q492.4[生物学—生理学]