Colonization Pattern of Azospirillum brasilense Yu62 on Maize Roots  

巴西固氮螺菌Yu62在玉米根的定植(英文)

作　　者：刘元[1] 陈三凤[1] 李季伦[1] 

机构地区：[1]中国农业大学生物学院

出　　处：《Acta Botanica Sinica》2003年第6期748-752,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家高技术研究发展计划"863"项目(2 0 0 1AA2 14 0 2 1);国家重点基础研究发展规划项目 (0 0 1CB10 890 4)~~

摘　　要：Plasmid pVK1001 which carried the gfp gene of GFPmut2, a mutant of GFP, was introduced into Azospirillum brasilense Yu62 by electroporation. Maize seedlings were inoculated with the GFP-labelled baeteria and grown gnotobiotically in flask with semi-solid agar medium. Observations were performed with confocal laser scanning microscopy (CLSM) and electron microscopy, respectively, at 8 d and 12 d after inoculation. Confocal laser scanning microscopy showed that A. brasilense Yu62 could penetrate into the cortex tissue, colonizing in the intercellular spaces of the parenchyma cells of the cortex tissue. Transmission and scanning electron microscopy (TEM) showed that the majority of the bacteria colonized on the root surface and only a minority of them resided in the root interior.将GFPmut2质粒中的gfp基因 (编码绿色荧光蛋白 )克隆到载体pVK10 0中 ,构建成重组质粒pVK10 0 1。将pVK10 0 1通过电转化方法导入到联合固氮菌巴西固氮螺菌Yu6 2中 ,获得GFP标记的巴西固氮螺菌Yu6 2菌株。用标记菌株接种限菌培养条件下生长的玉米 (农大 3318)幼苗 ,在接种后 8d、12d ,用激光共聚焦扫描显微镜进行观测 ,结果表明巴西固氮螺菌Yu6 2菌株能定植于玉米根部皮层的薄壁细胞间隙。用扫描电镜和超薄切片电镜观察表明 ,大多数细菌主要定植于根表 。

关 键 词：green fluorescent protein (GFP) Azospirillum brasilense Yu62 COLONIZATION confocal laser scanning microscopy ( CLSM) transmission electron microscopy (TEM) scanning electron microscopy (SEM) 

分 类 号：S182[农业科学—农业基础科学]