上海地区婴幼儿腹泻的轮状病毒分子流行病学研究  

Molecular epidemiologic survey of rotaviruses from infants and children with diarrhea in Shanghai

作　　者：曾玫[1] 朱启镕[1] 张又[2] 李国华[2] 陈冬梅[2] 丁雅馨[2] 钱渊[2] 

机构地区：[1]上海复旦大学附属儿科医院传染科,200032 [2]首都儿科研究所北京市感染与免疫中心实验室,100020

出　　处：《中华儿科杂志》2004年第1期10-15,共6页Chinese Journal of Pediatrics

基　　金：卫生部科研基金资助项目 (13 85 3 12 );北京市自然科学基金重点资助项目(795 10 0 2 )

摘　　要：目的　了解上海地区婴幼儿腹泻轮状病毒感染的分子流行病学特点。方法　收集复旦大学儿科医院 1999年 11月～ 2 0 0 1年 12月 ,住院的腹泻病儿童粪便标本 12 30份。采用聚丙烯酰胺凝胶电泳法检测轮状病毒基因组 ,用核酸杂交确定轮状病毒G血清型别。采用Kruskal Wallis非参数统计法对不同G血清型别的临床变量作分析。结果　 12 30份标本中轮状病毒基因阳性 4 93份(40 1% ) ,其中RNA长型 397份 (80 5 % ) ,短型 5 5份 (11 2 % ) ,混合型 18份 (3 7% ) ,未能分型 2 3份(4 7% )。 32 8份含有足量RNA的标本行RT PCR扩增VP7全基因 ,2 5 4份标本 (77 4 % )获得阳性产物 ,进一步作杂交分型显示G1血清型 14 1例 (5 5 5 % ) ,占第一位 ;G3血清型 70例 (2 7 6 % ) ,占第二位 ;G2血清型 2 4例 (9 4 % ) ,占第三位 ;混合感染血清型 16例 (6 3% ) ,占第四位 ;仅发现 1例G4型(0 4 % ) ,未分型 2例 (0 8% )。所有G2型均为电泳短型 ,而G1、G3、G4均为电泳长型。四种型别轮状病毒感染患儿的平均发病年龄以及发热峰值、热程、每天腹泻峰次数、腹泻天数、呕吐天数差异无显著意义。结论　A组轮状病毒是目前上海地区腹泻儿童的主要致病原。在不同的 3年中 ,轮状病毒的电泳型均以长型为主 ,流行血清型为G1 G3型 。Objective To investigate molecular epidemiologic features of rotaviruses circulating in Shanghai, China Methods Stool samples were collected from 1230 hospitalized children with community acquired and nosocomially acquired diarrhea in Children′s Hospital Affiliated to Fudan University between November 1, 1999 and December 31, 2001 Polyacrylamide gel electrophoresis (PAGE) was used to detect rotavirus genomic RNA and identify electropherotypes of group A rotavirus RNAs Reverse transcription polymerase chain reaction (RT PCR) was performed to amplify full length VP7 gene and dot blot hybridization was performed to identify rotavirus G serotypes using digoxigenin labelled variable regions from VP7 genes as probes These probes were amplified by PCR from recombinant plasmids containing full length G1, G2, G3 and G4 VP7 genes from rotavirus field strains detected in Beijing and digoxigenin labelled dUTP was integrated into the PCR products The Kruskal Wallis analysis of variance was employed to analyze whether there were significant differences in variables Results Out of 1230 samples investigated, 493 (40 1%) were group A rotavirus gene positive by PAGE, among which 397 (80 5%) showed long electropherotypes, 55 (11 2%) showed short electropherotypes, 18 (3 7%) showed mixed electropherotypes which suggested that the children were co infected by rotaviruses with different electropheoypes, 23 (4 7%) were non typable because of degradation of some of the genomic RNA fragments No group B or group C rotavirus was found RT PCRs were performed for 328 fecal specimens containing sufficient rotavirus RNAs and VP7 gene products were obtained from 254 (77 4%) samples Dot blot hybridization showed serotype G1 accounted for 55 5% (141) of these samples, serotype G3 accounted for 27 6% (70), serotype G2 accounted for 9 4% (24) , co infection by 2 rotaviruses with different G types accounted for 6 3% (16) , only 1 G4 was detected and 2 were non typable The genomic RNA patterns of al

关 键 词：上海 婴幼儿腹泻 轮状病毒分子 流行病学 病毒基因 

分 类 号：R725.1[医药卫生—儿科]