人抵抗素基因克隆和蛋白纯化  

Cloning of human resistin gene and its protein purification

作　　者：何祥梁[1] 何东华[2] 李芳萍[1] 黎锋[1] 程桦[1] 傅祖植[1] 

机构地区：[1]广州中山大学附属第二医院内分泌科,510120 [2]中山大学达安基因中心

出　　处：《中华内分泌代谢杂志》2004年第1期80-81,共2页Chinese Journal of Endocrinology and Metabolism

基　　金：中国博士后科学基金会资助 (2 0 0 2 0 31 2 96)

摘　　要：从人脂肪组织用RT PCR扩增抵抗素基因cDNA并测序 ,PCR产物亚克隆到T载体 ,再克隆到原核表达载体 ,表达质粒转化大肠杆菌并诱导表达 ,以Glutathin Sephrose 4B亲和层析柱纯化了目的融合蛋白。The human resistin cDNA from human adipose tissue was amplified by RT-PCR and sequenced, and cloned into a prokaryotic expression vector. The fusion protein was expressed by E.coli and purified by Glutathion-Sepharose 4B affinity chromatography.

关 键 词：抵抗素 基因克隆 蛋白纯化 测序 

分 类 号：R346[医药卫生—基础医学]