氦氖激光穴位照射对缺血缺氧新生大鼠脑神经元尼氏体与BDNF表达的影响  

Effect of He-Ne laser acupoint irradiation on Nissl bodies and expression of BDNF after hypoxia-ischemia in neonatal rat′s brain neuron

作　　者：刘伟[1] 吴爱群[2] 李宛青[3] 高艳[2] 侯晓强[4] 汤善均[2] 

机构地区：[1]中国协和医科大学基础医学院解剖与组织胚胎学系,北京100005 [2]郑州大学基础医学院人体解剖教研室 [3]郑州师范高等专科学校生命科学系 [4]郑州大学基础医学院物理教研室

出　　处：《解剖学研究》2003年第4期270-272,276,I001,共5页Anatomy Research

基　　金：河南省科技攻关项目 ( 0 2 110 43 80 0 )

摘　　要：目的　探讨氦氖激光穴位照射对新生鼠缺血缺氧性脑损伤的作用及其可能机制。方法　 4 2只 7d龄Wistar大鼠 ,随机分为 3组 :假手术对照组、缺血缺氧组、缺血缺氧后氦氖激光穴位照射组 ,常规饲养 2 2d后 ,取出左侧脑组织进行常规石蜡包埋、切片、尼氏染色和BDNF免疫组织化学染色。结果　氦氖激光穴位照射可明显改善缺血缺氧后脑神经元内尼氏体的脱失现象 ,增加缺血缺氧后脑神经元内和BDNF的表达。结论　氦氖激光穴位照射对缺血缺氧后脑组织具有神经保护效应 。Objective To study the effect and possible mechanism of He Ne laser acupoint irradiation treatment on hypoxic ischemic brain damage (HIBD). Methods Forty two 7 days old Wistar rats were randomly divided into three groups.Ⅰ: sham operation group; Ⅱ: hypoxia ischemia group; Ⅲ:He Ne laser acupoint irradiating group. 22 days later, left brains were made into paraffin sections, processed for Nissl staining,BDNF immunohistochemistry staining.Results He Ne laser acupoint irradiation can improve the decrease of Nissl bodies in brain neuron, increased the BDNF positive remarks in hypoxia ischemia brain neurons. Conclusions He Ne laser acupoint irradiation have nruroprotective function ,which maybe relate to the expression of BDNF.

关 键 词：氦氖激光 穴位照射 新生儿缺血缺氧性脑损伤 新生大鼠 脑神经元尼氏体 BDNF 免疫组织化学 

分 类 号：R454.2[医药卫生—治疗学]