电针对新生大鼠缺血缺氧后脑组织ChAT和BDNF表达的影响  

Influence of electroacupuncture on expression of ChAT and BDNF after hypoxia-ischemia in neonatal rat brain

作　　者：李宛青[1] 刘伟[2] 吴爱群[2] 高艳[2] 万国兰[3] 汤善钧[2] 

机构地区：[1]郑州师范高等专科学校生命科学系,郑州450015 [2]郑州大学基础医学院人体解剖教研室,郑州450052 [3]郑州大学第三附属医院儿科,郑州450052

出　　处：《解剖学研究》2003年第4期295-297,305,I005,共5页Anatomy Research

基　　金：河南省科技攻关项目 ( 0 2 110 43 80 0 )

摘　　要：目的　探讨电针治疗缺血缺氧性脑病的远期疗效及可能机制。方法　 4 2只出生 7d的Wistar大鼠 ,随机分为 3组 :假手术对照组、缺血缺氧组、缺血缺氧后电针治疗组 ,常规饲养 2 2d后 ,取左侧脑组织切片进行尼氏染色、ChAT和BDNF免疫组织化学染色。结果　电针可明显改善缺血缺氧后脑组织神经元内尼氏体脱失现象 ,增加缺血缺氧脑组织内ChAT和BDNF阳性细胞表达。结论　电针对缺血缺氧后脑组织具神经保护效应 。Objective To probe the influence of electroacupuncture on expression of ChAT and BDNF after hypoxia ischemica in neontal rat brain to find the effective mechanism of possibility.Methods Forty two 7 days old Wistar rats were randomly divided into three groups.Ⅰ:sham operation group;Ⅱ:Hypoxia is chemia group;Ⅲ:Electroacupuncture group.22 days later,The left side brains were made into paraffin sections,processed for Nissl staining,ChAT and BDNF immunohistochemistry staining.Results EA could improve the loss of Nissl bodies,increase the ChAT and BDNF positive remarks in hypoxia ischemia brain.Conclusion EA could protect baain against hyproxic ischemic damage,which might be the result of the increase of BDNF expression.

关 键 词：电针 新生儿缺血缺氧性脑损伤 脑源性神经营养因子 胆碱乙酰转移酶 神经保护效应 自由基 

分 类 号：R245.97[医药卫生—针灸推拿学]