HPLC法测定人全血中西罗莫司的浓度  

Determination of sirolimus concentration in human whole blood by HPLC

作　　者：时颖华[1] 李中东[1] 施孝金[1] 钟明康[1] 

机构地区：[1]复旦大学附属华山医院临床药学研究室,上海200040

出　　处：《中国临床药学杂志》2004年第2期76-78,共3页Chinese Journal of Clinical Pharmacy

摘　　要：目的 :建立全血中西罗莫司 (SRL)的HPLC测定方法。方法 :以 32 去甲氧基雷帕霉素为内标 ,全血样品先破坏RBC ,取离心后的上清液用固相萃取处理。采用KromasilC18(15 0mm× 4 .6mm ,5 μm)柱 ,柱温 5 5℃ ,紫外检测波长 2 78nm ,流动相为乙腈 甲醇 水 (4 5∶2 8∶2 7,V/V/V) ,测定SRL浓度。结果 :全血中内源性杂质对样品测定无干扰。SRL在 2 .5～ 6 0 μg·L-1范围内线性关系良好 (r=0 .9990 )。日内和日间RSD均 <9.2 % ,全血样品多次冻融及提取后在 2 4h内稳定性良好。结论 :该方法操作简便、灵敏、准确 。AIM: To establish a HPLC method for determination of concentration of sirolimus(SRL) in whole blood . METHODS: HPLC analytical column was Kromasil C 18(150 mm×4.6 mm,5 μm); column temperature 55℃, ultraviolet detection wavelength 278 nm; mobile phase consisted of acetonitrile-methanol-water(45∶28∶27,V/V/V). The red cells in whole blood sample were firstly destroyed, after centrifugation, the supernatant was extracted using solid-phase column, and SRL was determined using 32-desmethoxy rapamycin as internal standard. RESULTS: The blank whole blood did not interfere with the determination of SRL and internal standard. It was good linear relationship(1/c 2 weighted) between peak area ratio of SRL to internal standard and c(r=0.999 0) within the range of 2.5-60 μg·L -1. The precision of within-run and between-run was good. CONCLUSION: The method is simple, sensitive, accurate and suitable for therapeutic drug monitoring and clinical pharmacy research of SRL.

关 键 词：HPLC法 测定方法 人 全血中西罗莫司 浓度 高效液相色谱 固相萃取 

分 类 号：R96[医药卫生—药理学]