聚合酶链反应-探针杂交-酶显色用于结核分支杆菌的检测  

Detection of mycobacterium tuberculosis with PCR-Probe Hybridization-ELISA

作　　者：熊国亮[1] 雷建平[1] 张慧慧[1] 刘珍琼[1] 周萍珍[1] 

机构地区：[1]江西省肺科医院检验科,江西南昌330006

出　　处：《中国实验诊断学》2004年第2期150-152,共3页Chinese Journal of Laboratory Diagnosis

基　　金：江西省卫生厅资助项目 (编号 :2 0 0 0 110 3 )

摘　　要：目的　评价聚合酶链反应 探针杂交 酶显色方法 ,检测结核分支杆菌。方法　收集 131例活动性肺结核患者和 30例非结核呼吸系疾病患者的痰标本以双盲法进行痰涂片抗酸染色检查 ,结核菌培养、PCR 探针杂交 酶显色法、PCR 琼脂糖凝胶电泳技术检查对比。结果　涂片法、培养法、PCR 探针杂交 酶显色法的敏感性分别为 2 6 72 %、4 1 2 2 %、70 2 3% ,PCR 探针杂交 酶显色法的敏感性显著高于培养法和涂片法 (P <0 0 1)。PCR 探针杂交 酶显色法和PCR琼脂糖凝胶电泳法的敏感性分别为 70 2 3%、70 99% ,特异性分别为 96 6 7%、86 6 7% ,前者特异性比后者高 ,且PCR 探针杂交 酶显色法只检出结核分支杆菌和牛型分支杆菌。结论　PCR 探针杂交 酶显色技术检测结核分支杆菌灵敏度高 ,特异性好 ,简便快速 ,同时能筛检非结核分支杆菌。因此 。Objective To evaluate the value of PCR-probe hybridization-ELISA for detection of mycobacterium tuberculosis(M.tuberculosis).Methods The sputum specimens taken from 131 patients with active pulmonary tuberculosis and 30 patients with respiratory system diseases other than tuberculosis, were detected double-blindly with thick smears acid-fast staining, culture, PCR-probe hybridization-ELISA,PCR-agar gel electrophoresis(PCR-AGE) technique, And the results were compared.Results The sensitivities of smears, cultures and PCR-probe hybridization-ELISA were 26 72%, 41 22%, 70 23%,respectively.The sensitivity of PCR-probe hybridization-ELISA was obviously higher than that of the other two methods( P <0 01).The sensitivity was 70 23% by PCR-probe hybridization-ELISA and 70 99% by PCR-AGE. The specificity was 96 67% by PCR-probe hybridization-ELISA and 86 67% by PCR-AGE, The specificity of the former was higher than that of the latter, PCR-probe hybridization-ELISA can only detect M .tuberculosis and M. bovis.Conclusion PCR-probe hybridization-ELISA is a more sensitive, more specific, simpler, more rapidly method for detection of M. Tuberculosis and can identifymycobacterium tuberculosis with non-mycobacterium tuberculosis. PCR-probe hybridization-ELISA is one of the effective methods in clinical application.

关 键 词：聚合酶链反应 探针杂交 酶显色 结核分支杆菌 诊断 PCR 

分 类 号：R446.5[医药卫生—诊断学]