神经鞘氨醇磷酸胆碱对原代培养人真皮成纤维细胞DNA合成影响  

Effect of DNA synthesis of primerry cultures of human dermal fibroblasts by Sphingosylphosphorylcholine

作　　者：朱明姬[1] 蒋忠民[1] 丛宪玲[1] 丛建军 李增勋[3] 陈远耀[4] 

机构地区：[1]吉林大学中日联谊医院皮肤科,长春130033 [2]通话市人民医院 [3]大韩民国忠南大学医科大学医院皮肤科 [4]吉林大学基础医学院病理教研室

出　　处：《中国实验诊断学》2004年第2期145-146,共2页Chinese Journal of Laboratory Diagnosis

基　　金：大韩民国保健福祉部资助课题 ( 2 0 0 1)

摘　　要：目的　观察神经鞘氨醇磷酸胆碱 (Sphingosylphosphorylcholine ,SPC)对原代培养人真皮成纤维细胞DNA合成的影响。方法　利用儿童包皮组织行人真皮成纤维细胞原代培养 ,采用不同浓度SPC处理培养的细胞 ,测定3H TdR掺入量。结果　SPC浓度为 2 μM时 ,3H TdR掺入量明显高于对照组 (P <0 0 5 ) ,5 μM时3H TdR掺入量达高峰 ,而后逐渐降低 ,2 0 μM时3H TdR掺入量为最低。结论　在一定浓度范围内SPC能明显促进原代培养人真皮成纤维细胞DNA的合成 ,在人类皮肤创伤愈合的治疗领域中 。Objective To investigate Effect of DNA synthesis of primerry cultures of human dermal fibroblasts by Sphingosylphosphorylcholine.Methods Measure the [ 3H]thymidine incorporation of primerry cultures of human dermal fibroblasts after Dose-dependent Stimulation of SPC.Results At the concentration of 2 μM SPC, the DNA [ 3H]thymidine incorporation was much higher than the control, Gave 5 μM SPC the [ 3H]thymidine incorporation reached the highest level. But when the concentration of SPC was 20 μM the [ 3H]thymidine incorporation decreased to the lowest level.Conclusion when the concentration is at 2～10 μM SPC greatly stimulates DNA synthesis of primerry cultures of human dermal fibroblasts, But when the concentration is 20 μM, SPC has cytotoxity to fibroblasts.

关 键 词：神经鞘氨醇磷酸胆碱 SPC 真皮成纤维细胞 DNA合成 细胞培养 原代培养 

分 类 号：R75105[医药卫生—皮肤病学与性病学]