不同年龄大鼠可溶性脑蛋白聚糖的比较分析  

Comparative analysis on soluble rat brain proteoglycan of different ages

作　　者：梁雪清[1] 田梦玉[1] 

机构地区：[1]西安交通大学医学院生化教研室,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2004年第2期118-120,123,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目 (No.390 70 2 4 0 )

摘　　要：目的　探讨可溶性脑蛋白聚糖 (proteoglycan ,PG)与脑发育的关系。 方法　纯种SD大鼠分为 3组 :初生组、青年组和近老年组。用盐酸胍分别提取各组大鼠的可溶性脑PG ,提取后的脑PG经DEAE sephacel柱层析分离纯化 ,再用Sepharose 4B柱层析分析其分子大小 ,用双向电泳法分析其糖胺多糖 (glycosaminoglycan ,GAG)的组成。 结果　可溶性脑PG的提取量随增龄而降低 ;可溶性脑PG按分子大小分为 4个部分 ,大分子集聚型硫酸软骨素蛋白聚糖(CSPG)的比例随增龄而增高 ,但其中所含透明质酸 (HA)的比例随增龄而显著降低 ;含硫酸皮肤素蛋白聚糖 (DSPG)的小分子部分也随增龄呈下降趋势。结论　可溶性脑PG随动物年龄而发生一定的变化 。Objective To analyze the relationship between soluble brain proteoglycan (PG) and brain development. Methods SD rats were divided into three groups: new-born, young adult and near-old groups. Soluble brain PGs of each group were extracted with 4 mol·L -1 guanidine HCl and purified by DEAE-sephacel chromatography, their molecular sizes were identified by Sepharose 4B chromatography, and their GAG compositions were characterized by two dimensional electrophoresis. Results The extraction amounts of soluble brain proteoglycans were decreased with age; soluble brain PGs could be divided into four fractions according to molecule size. The percentage of the large size CSPG aggregate increased with age, but its HA declined. The percentage of the small sized fraction containing DSPG declined with age increase. Conclusion Soluble brain PGs change with age and are related to brain development.

关 键 词：大鼠 可溶性脑蛋白聚糖 脑发育 初生 青年 老年 

分 类 号：R338[医药卫生—人体生理学]