底物加料方式对氢化可的松发酵的影响  

Effect of the Pattern of Substrate Addition on Hydrocortisone Fermentation

作　　者：陈泮成[1] 卢文玉[1] 郭亚文[1] 王敏[1] 杜连祥[1] 

机构地区：[1]天津科技大学食品科学与生物工程学院,天津300222

出　　处：《中国医药工业杂志》2004年第4期206-209,共4页Chinese Journal of Pharmaceuticals

基　　金：天津市重大科技攻关项目(023180211);教育部科技研究重点项目(天津01004)

摘　　要：考察了甾体11b-羟基化过程中,底物17a,21-二羟基孕甾-4-烯-3,20-二酮-21-醋酸酯(2)投料时间、投料量及pH对细胞色素P-450(3)诱导和氢化可的松(1)产量的影响。在此基础上提出了生产1的诱导发酵改进方法:菌体生长至16h投入2 0.3g/L,培养至24h补加2 0.7g/L,培养至32h调至pH6.5,以后每12h调节一次。结果表明,在发酵周期不变(68h)的情况下,3蛋白量和1产量分别比一次投料法提高了11.2%和12.7%。The feeding time and concentration of 17a,21-dihydroxypregn-4-ene-3,20-dione-21-acetate(2)and thepH value of the environment which affected the induction of cytochrome P-450 and the yield of hydrocortisone during the11a-hydroxylation of steroids were investigated. The optimal fermentation conditions for induction were as follows: 0.3g/Land 0.7g/L 2 were fed at 16h and 24h after inoculation, respectively; adjusted the pH value to 6.5 at 32h and then regulatedto 6.5 every 12h. It was showed that the content of cytochrome P-450 protein was increased by 11.2% and the yield ofhydrocortisone was increased by 12.7% over the single feeding method.

关 键 词：新月弯孢霉 细胞色素P-450 氢化可的松 

分 类 号：TQ467.6[化学工程—制药化工]