Expressional Analysis of an EREBP Transcription Factor Gene OsEBP-89 in Rice  

作　　者：HuiSHEN Zong-YangWANG 

机构地区：[1]TheStateKeyLaboratoryofPlantMolecularGenetics,InstituteofPlantPhysiology＆Ecology,ShanghaiInstitutesforBiologicalSciences,theChineseAcademyofSciences,Shanghai200032,China

出　　处：《Acta Biochimica et Biophysica Sinica》2004年第1期21-26,共6页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the High Technology Department Program of China(No.2002AA2Z1003) ; the Major State Basic Research Development Program of China (973 Program) (No. G1999011604)

摘　　要：OsEBP-89 gene encodes an ethylene responsive element binding protein (EREBP) transcription factor from rice (Oryza saliva). Northern blot analysis revealed that OsEBP-89 was expressed in root, stem, seeds, flowers and leaves. Histochemical assay showed that β-glucuronidase (GUS) was expressed mainly in phloem of vascular tissues of the root and stem transition region (RST), basal part of sheath roots, stem node and basal part of adventitious roots, also in endosperm of seeds in transgenic rice harboring OsEBP-89/GUS construct (pNSG). A sequence region from -279 to -97 was found to play an important role for OsEBP-89 gene expression through promoter deletion assay. The possible function of OsEBP-89 gene was discussed.OsEBP-89 gene encodes an ethylene responsive element binding protein (EREBP) transcription factor from rice (Oryza saliva). Northern blot analysis revealed that OsEBP-89 was expressed in root, stem, seeds, flowers and leaves. Histochemical assay showed that β-glucuronidase (GUS) was expressed mainly in phloem of vascular tissues of the root and stem transition region (RST), basal part of sheath roots, stem node and basal part of adventitious roots, also in endosperm of seeds in transgenic rice harboring OsEBP-89/GUS construct (pNSG). A sequence region from -279 to -97 was found to play an important role for OsEBP-89 gene expression through promoter deletion assay. The possible function of OsEBP-89 gene was discussed.

关 键 词：OsEBP-89 gene RICE EREBP subfamily transcription factor tissue expression 

分 类 号：S511.01[农业科学—作物学]