类风湿关节炎患者Th1/Th2细胞因子分泌模式与疾病活动程度的关系  

Relationship between Th1/Th2 cytokine secretion patterns and disease activity in patients with rheumatoid arthritis

作　　者：张浩[1] 董书魁[1] 李雪丽[1] 王清娟[1] 田京霞[1] 

机构地区：[1]解放军第二炮兵总医院检验科,北京市100088

出　　处：《中国临床康复》2003年第30期4068-4069,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:用全血在单个细胞水平上研究类风湿关节炎(rheumatoidarthritis,RA)患者Th1/Th2细胞因子分泌模式及其与疾病活动程度的关系。方法:用三色流式细胞术检测20例活动期RA患者的外周血单个核细胞及10例正常人的PBMC细胞内细胞因子干扰素-γ(interferon-γ,IFN-γ)和白细胞介素-4(interleukin-4,IL-4)的表达情况。结果:RA患者外周血IFN-γ+细胞百分率及IFN-γ+/IL-4+细胞比值比健康人明显增高犤分别为(45.8±10.1)%和(15.2±8.5)%,t=9.07,P<0.01和(10.6±3.8)%和(5.5±3.1)%,t=5.67,P<0.01犦,IFN-γ+/IL-4+比值与患者Stoke指数呈正相关(r=0.8610,P<0.01)。结论:RA患者外周血中细胞因子分泌模式朝Th1偏移,IFN-γ+/IL-4+比值与疾病活动性相关,Th1/Th2平衡在RA发病机制及疾病进程中起重要作用。AIM:To study the relationship between the Th1/Th2 cytokine secretion patterns and the disease activity at the single cell level in the peripheral blood of rheumatoid arthritis (RA) patients. METHODS:Expressions of single nuclear cells of peripheral blood in 20 patients with active RA and PBMC intracellular cytokines such as IFN γand IL 4 in 10 healthy individuals were examined by three colour flow cytometry. RESULTS:The percentage of IFN γ+cells and IFN γ+/IL 4+ratio were significantly increased in RA patients compared with healthy individuals[(15.8±10.1)%vs(15.2±8.5)%,t=9.07,P< 0.01,and(10.6±3.8)%vs( 5.5±3.1)%,t=5.67,P< 0.01,respectively].The IFN γ+/IL 4+ratio in RA patients correlated significantly with the Stoke Index (r=0.893,P< 0.01).

关 键 词：类风湿关节炎 TH1/TH2 细胞因子 分泌模式 疾病活动程度 三色流式细胞术 检测 

分 类 号：R593.22[医药卫生—内科学]