创伤性休克大鼠血浆肾上腺髓质素变化的实验研究  

EXPERIMENTAL STUDY OF CHANGES IN PLASMA ADRENOMEDULLIN IN RATS WITH TRAUMATIC SHOCK

作　　者：孙高斌[1] 黄宗海[1] 孙英刚[1] 厉周[1] 林洪武[1] 宋慧娟[1] 

机构地区：[1]第一军医大学珠江医院

出　　处：《解放军医学杂志》2003年第8期690-691,共2页Medical Journal of Chinese People's Liberation Army

基　　金：广东省自然科学基金资助课题 (0 0 1 0 4 8)

摘　　要：探讨创伤性休克时大鼠血浆肾上腺髓质素 (AM)的变化特点及其机制。将 30只SD大鼠分为对照组、休克未复苏组和休克复苏组 ,对照组 10只麻醉后插管 ;2 0只SD大鼠制作创伤性休克动物模型 ,双侧股骨干砸伤后并经股动脉放血至平均动脉压 35～4 5mmHg。休克复苏组血压维持 30min,然后回输失血和等量的林格液。观察休克前后血浆AM浓度的动态变化。结果显示 ,大鼠创伤性休克后 ,血浆AM水平明显高于对照组 ,休克早期高于休克晚期 ,未复苏组高于复苏组。提示AM在创伤性休克发生发展中起重要的调节作用 ,休克早期AM血浆水平明显升高 ,并且达到高峰 。To observe the changes in plasma adrenomedullin (AM) and its possible mechanism of pathogenesis of traumatic shock, 30 Sprague Dawley rats were randomly divided into control group, traumatic shock without resuscitation group, and traumatic shock with resuscitation group. Ten control rats were intubated after anesthesia only. Traumatic shock was reproduced in 20 rats by fracturing shafts of both femurs and bleeding to a mean arterial pressure of 35～45mmHg via femoral artery. Hypotension of rats with traumatic shock with resuscitation group was maintained 30min, and then the bled blood was returned, followed by an infusion with same volume of Ringer's solution. Plasma levels of adrenomedullin(AM) were assayed before and after shock. The data showed that plasma levels of AM were significantly increased after shock, and they were higher in the early period than late period. They were also higher in rats without resuscitation than that in rats of the resuscitation group. The results suggested that plasma levels of AM were significantly increased at an early in rats with traumatic shock, implicating that it might play an important role in the development of traumatic shock. Measures taken to decrease the levels of AM at this period might be helpful to improve the result of resuscitation of traumatic shock in rats

关 键 词：休克 创伤性 肾上腺髓质素 大鼠 

分 类 号：R605.971[医药卫生—急诊医学]