CPT-环磷酸腺苷对视网膜节细胞再生的影响  

EFFECTS OF CPT-cAMP ON THE REGENERATION OF THE RETINAL GANGLION CELLS IN ADULT HAMSTERS

作　　者：朱永红[1] 李海标[1] 

机构地区：[1]中山大学中山医学院组织学与胚胎学教研室,广州510080

出　　处：《解剖学报》2003年第5期458-461,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 (39870 2 6 6 ) ;广东省自然科学基金资助项目 (980 0 96 )

摘　　要：目的　探讨玻璃体内插入外周神经和玻璃体内注射CPT 环磷酸腺苷对轴突损伤的视网膜节细胞再生的影响。　方法　 2 0只成年金黄地鼠随机分 4组 ,每组 5只。切断视神经 (ON)并缝接坐骨神经为再生对照组(attachedgraft,AG) ;ON切断并缝接坐骨神经后再于玻璃体内注射CPT cAMP和 或插入一小段自体坐骨神经 (SN)为实验组。采用逆行示踪标记术 ,观察视网膜平铺片节细胞数。　结果　 1 对照组 (AG) ,视网膜再生的节细胞数为 :142 8± 2 84个 ;2 外周神经组 (AG +SN) :每个视网膜再生的节细胞平均数为 2 2 2 0± 415个 ;3 CPT cAMP组 (AG+cAMP) :每个视网膜再生的节细胞平均数为 2 175± 36 4个 ;4 外周神经和CPT cAMP联合组 (AG +cAMP +SN) :每个视网膜再生的节细胞平均数为 42 5 5± 82 0个 ;其中 ,AG +SN、AG +CPT组同AG组相比存在差异显著性 (P <0 0 1) ;AG +CPT +SN组同AG、AG +CPT和AG +SN组相比均有差异显著性 (P <0 0 1)。　结论　研究结果提示玻璃体内注射CPT +cAMP或联合应用外周神经和CPT cAMP可大幅提高受损视网膜节细胞的再生。Objective To investigate the effects of transplantation of sciatic nerve(SN) and injection of 8-(4-Chlorophenylthio)-cAMP(CPT-cAMP) into the viteous on axotomized retinal ganglion cells(RGCs) in adult hamsters. Methods Twenty adult golden hamsters were ramdomly divided into 4 groups.Optic nerve(ON) was transected and a segment of autologous sciatic nerve(attached graft,AG) was removed and sutured to the proximal stump of ON in regenerating control group(AG group).The animals in experimental groups were further treated with CPT-cAMP injection and/or implantation of a short of segment sciatic nerve(SN) intravitrously.Fluorescent retrograde labeling method and quantitative anatomical techniques were used to measure the number of RGCs. Results 1.In control (AG) retinas,the mean number of regenerating RGCs was 1*!428±284/retina.2.In AG+SN group,the mean number of regenerating RGCs was 2*!220±415/retina.3.In AG+cAMP group,the mean number of regenerating RGCs was 2*!175±364/retina.4.The mean number of regenerating RGCs in AG+cAMP+SN group were 4*!255±820/retina.AG+SN,AG+cAMP groups were significantly different from AG group(P<0.01).AG+cAMP+SN group was significantly different from that of AG,AG+cAMP and AG+SN groups(P<0.01).Conclusion Our results indicated that SN combined with cAMP had significant effects in promoting the regeneration of RGCs after axotomy.

关 键 词：CPT-环磷酸腺苷 视网膜节细胞再生 坐骨神经 金黄地鼠 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]