促性腺激素释放激素类似物对大鼠胃肠道5-羟色胺分泌的影响  

THE EFFECT OF GnRH-A IN THE STOMACH ON THE SECRETION OF 5-HT IN THE DIGESTIVE TRACT OF RATS

作　　者：朱晓东[1] 王玉珍[1] 刘晓宁[2] 黄威权[2] 

机构地区：[1]中国科学技术大学生命科学院分子生物学与细胞生物学系,合肥230027 [2]第四军医大学组织学与胚胎学教研室,西安710033

出　　处：《解剖学报》2003年第5期518-521,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 (39770 388)

摘　　要：目的　研究GnRH对胃肠道内 5 HT分泌的影响。　方法　胃腔直接注射GnRH类似物阿拉瑞林(AlarelinGnRH A)以模拟外分泌产生的GnRH ,并以免疫组织化学、高压液相色谱电化学检测 (HPLC ECD)的方法对阿拉瑞林刺激后胃及十二指肠内 5 HT免疫反应细胞、血清中 5 HT含量进行检测。　结果　胃腔内注射GnRH A后 ,大鼠胃及十二指肠内 5 HT免疫反应阳性细胞密度显著增多 ,但血清中 5 HT含量显著减少。　结论　外分泌的GnRH对于 5 HT的释放起明显抑制作用 ,但对 5 HT的合成可能不产生影响。Objective To study the effect of GnRH on the secretion of 5-HT in the stomach. Methods The GnRH-A Alarelin was directly injected into the stomach of rats to observe the change of density of 5-HT immunoreactive positive cell in the stomach and small intestine by immunohistochemical SABC method and 5-HT level in the circulating blood by HPLC-ECD, Results Compared with the control group,which was injected with saline into the stomach,the density of immunoreactive positive cell was significantly increased in the stomach and small intestine in the GnRH-A treatment group;while 5-HT level in the circulating blood was significantly reduced in the experimental group.Conclusion GnRH can inhibit the releasing of 5-HT but not change its synthesis.

关 键 词：促性腺激素释放激素 大鼠 胃肠道 5-羟色胺 分泌 免疫组织化学 高压液相色谱电化学检测 

分 类 号：R333.2[医药卫生—人体生理学]