大鼠胚胎神经干细胞的分离培养及鉴定  

Separation,culture and identification of neural stem cells from rat embryo

作　　者：张旭静[1] 钱国正[2] 范柳[1] 王春安[3] 王桂清[1] 

机构地区：[1]上海市脑血管病防治研究所,上海200433 [2]第二军医大学长海医院生物医学工程研究室,上海200433 [3]基础医学部生理学教研室

出　　处：《第二军医大学学报》2003年第5期521-523,共3页Academic Journal of Second Military Medical University

基　　金：上海市科委科技发展基金(014119006;014119009).

摘　　要：利用无血清培养和细胞克隆技术从孕鼠(14 d)胚胎中分离出神经干细胞,并进行传代培养,采用免疫细胞化学技术检测神经巢蛋白和成熟脑细胞特异性抗原神经元特异性烯醇化酶(neuron specific enolase,NSE)、胶质纤维酸性蛋白(glialfibrillary acidic protein,GFAP)的表达,同时采用H-E染色法鉴定神经细胞类型。证实从大鼠胚胎大脑皮质分离出的细胞具有连续的克隆能力,并表达神经巢蛋白,分化的细胞表达神经元和神经胶质细胞的特异性抗原,为中枢神经系统的干细胞。This study is to set up a method for separating,culturing and identifying neural stem cells from cortex in rat embryo,observing their characteristics of multipotentiality and differentiation in vitro. By using serum-free cell culturing and clone technology,the stem cells were isolated,cultured and passaged from cortex in 14-day old rat embryo. The expression of Nestin antigen of brain cells were investigated by immunocytochemistry and H-E staining. It proved that cells separated from cortex in rat embryo possessed the potential to form clones and express nestin special antigen,the differentiated cells could express special antigen of neurons,astrocytes,and oligodendrocytes,they were stem cells of central nervous system.

关 键 词：大鼠 胚胎 神经干细胞 细胞培养 细胞鉴定 细胞分离 

分 类 号：R338[医药卫生—人体生理学]