自身骨髓瘤抗原致敏树突细胞介导特异性CTL反应的研究  

The research of specific cytotoxicity T lymphocyte response mediated by myeloma antigen-pulsed dendritic cells

作　　者：邓晓辉[1] 翁霞[1] 梁辉[1] 陈琳军[1] 马静秋[1] 余金妹[1] 

机构地区：[1]上海第二医科大学新华医院血液科,上海200092

出　　处：《中国免疫学杂志》2004年第4期241-243,共3页Chinese Journal of Immunology

基　　金：上海市科技发展基金资助项目 (批准号 :41190 2 4)

摘　　要：目的 :研究多发性骨髓瘤 (MM)患者肿瘤冻融物致敏的树突细胞 (DC)能否诱导特异性细胞毒T淋巴细胞(CTL)反应。方法 :用MM患者骨髓CD34+ 细胞诱生DC。将MM患者骨髓瘤冻融物冲击致敏DC。MTT法检测骨髓瘤抗原致敏及非致敏DC诱导的自身T细胞对不同靶细胞 (患者骨髓瘤细胞、K5 6 2细胞 )的杀伤率。结果 :骨髓瘤冻融物致敏DC诱导的自身T细胞对患者骨髓瘤细胞的杀伤远大于对K5 6 2细胞的杀伤。结论 :患者骨髓瘤冻融物致敏的DC能有效诱导自身T细胞特异性抗瘤免疫。Objective:To explore the specific cytotoxic T lymphocyte(CTL) response induced by DC pulsed with tumor lysates from multiple myeloma(MM) patients.Methods:Dendritic cell (DC) were cultured by CD34 + cells from bone marrow of MM patients. DCs were pulsed with myeloma lysates from MM. Killing rates of autologous T lymphocytes induced by DC pulsed with or without myeloma antigen on different target cells(MM cells,K562 cells) were measured by MTT method. Results:Autologous T lymphocytes induced by DCs pulsed with myeloma lysates had stronger killing effect on myeloma cells than that on K562 cells. Conclusion:DCs pulsed with myeloma lysates from MM patient can effectively induce autologous T cells specific anti-tumor immunity.

关 键 词：多发性骨髓瘤 树突细胞 肿瘤冻融物 肿瘤免疫 

分 类 号：R392.12[医药卫生—免疫学]