青藤碱对T淋巴细胞活化及TH1类细胞内细胞因子表达的影响  

Effects of sinomenine on cell activation and intracellular Th1 type cytokines expression of T lymphocytes

作　　者：李晓娟[1] 王培训[1] 刘良[2] 陈光星[3] 肇静娴[4] 曾耀英[4] 陈纪藩[3] 

机构地区：[1]广州中医药大学免疫研究室,广州510405 [2]香港浸会大学中医药学院 [3]暨南大学移植与免疫中心国家教育部重点实验室,广州510632 [4]广州中医药大学第一附属医院,广州510405

出　　处：《中国免疫学杂志》2004年第4期249-252,258,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金重点资助项目 ( 3 993 0 2 0 )

摘　　要：目的 :深入研究青藤碱对T淋巴细胞Th1类细胞因子表达的影响。方法 :分离小鼠淋巴结细胞 ,加入不同浓度的青藤碱作用 1小时后 ,加多克隆刺激剂PDB和离子霉素 ,继续培养 4小时后收获细胞 ,进行细胞内细胞因子染色 ,以流式细胞术对T细胞Th1类细胞因子TNF γ、炎症性细胞因子TNF α分子表达情况进行分析 ;同时 ,观察药物对T细胞活化早期标志CD6 9表达的影响 ;结果 :10 0 0 μmol L(32 9 2 4 μg ml)青藤碱能够抑制CD6 9表达 ,2 0 0 μmol L青藤碱对CD6 9表达无影响 ;2 0 0、10 0 0、2 0 0 0 μmol L青藤碱能够剂量依赖性地显著降低T细胞内细胞因子TNF γ、TNF α分子表达。 结论 :抑制T细胞活化异常可能是青藤碱治疗类风湿关节炎免疫药理机制之一 ,此抑制效应可能主要不是通过影响PKC而是与影响钙离子依赖的T细胞活化信号传导途径相关。Objective:To study deeply on the effects of sinomenine on Th1 type cytokines expression of T lymphocytes.Methods:The authors isolated and cultured the mesenteric lymph nodes of mice in vitro.The lymphocytes were stimulated with PDB and inomycin after added sinomenine in different concentrations for 1 hour.After 4 hours stimulation,cells were collected and stained intracellular cytokines with the dying regents,then the expression of Th1 type cytokine IFN-γ,pro-inflammatory cytokine TNF-α of T lymphocytes were analyzed by flow cytometry.And also observed the effects of drugs on the expression of CD69,an early marker of T cell activation.Results:1 000 μmol/L(329.4 μg/ml)sinomenine can downregulate CD69 expression of T lymphocytes while 200 μmol/L sinomenie show no effect on it.200,1 000,2 000 μmol/L sinomenine can obviously reduce the expression of intracellular cytokines such as IFN-γ,TNF-α in dose-dependent manner.Conclusion:One of the immunological mechanisms of sinomenine to treat rheumatoid arthritis may due to the inhibitory effects on abnormal T lymphoctytes activation.The inhibitory effects of sinomenine may mainly be relative to the inhibitory effects on Ca 2+ -dependant signal pathway of T lymphocytes activation but not PKC pathway.

关 键 词：青藤碱 细胞因子 TH1细胞 类风湿关节炎 

分 类 号：R392.12[医药卫生—免疫学]