丙酮酸氧化酶法在献血者血液筛查中的应用分析  

Evaluation of Pyruvate Oxidase Method to Detect ALT in Blood Donor Screening

作　　者：范泽旭[1] 

机构地区：[1]河南省南阳市中心医院检验科,南阳市473009

出　　处：《医药论坛杂志》2004年第6期12-13,15,共3页Journal of Medical Forum

摘　　要：目的　探讨丙酮酸氧化酶法检测丙氨酸氨基转移酶 (ALT)这一方法在献血者血液筛查中的适用性。方法　分别用丙酮酸氧化酶法、赖氏法和酮体粉法检测 5 2 78例体检标本 ,比较各种方法的敏感性和精密度。结果　丙酮酸氧化酶法的初筛阳性率为 7 0 7% ,复验阳性率为 1 97% ,CV <5 %。结论　酶法ALT检测可实现试验的自动、微量、快速、结果传输的网络化 ,且能够保存每份样品的原始记录 ,便于查询。Objective To evaluate using pyruvate oxidase method to detect alanine aminotransferase(ALT) in blood donor screening.Methods The ALT value of 5278 plasma samples were detected using pyruvate oxidase method,Reitman's method,and acetone body powder method respectively.Sensibility and repeatability were compared between those methods.Results First positive rate of pyruvate oxidase method is 7.07% and repeated positive rate of pyruvate oxidase method is 1.97%,CV<5%.Conclusion Using pyruvate oxidase method to detect ALT can be automatic,minim,rapidly,and original data can be easily saved and retrieved.Pyruvate oxidase method is suitable to be used in donor screening in blood bank.

关 键 词：丙酮酸氧化酶法 献血者 血液筛查 应用分析 

分 类 号：R446.11[医药卫生—诊断学]