胶原基组织工程支架对成纤维生长因子的控制释放与组织再生功能(英文)  

Immobilization of the basic fibroblast growth factor on collagen-based tissue engineering scaffold and tissue regeneration

作　　者：杨洪义[1] 颜永年[1] 熊卓[1] 张其清[2] 

机构地区：[1]清华大学机械系激光快速成形中心,北京市100084 [2]中国医学科学院生物医学工程研究所,天津市300192

出　　处：《中国临床康复》2004年第11期2164-2165,共2页Chinese Journal of Clinical Rehabilitation

基　　金：国家重点基础研究发展规划"九七三"项目基金资助(G199054309-4)~~

摘　　要：目的:通过将碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)与胶原基组织工程支架进行复合,实现对bFGF的控制释放,从而更有效的实现组织再生功能。方法:采用ELISA酶联免疫试剂盒对溶出的bFGF进行定量分析,考察支架对bFGF的短期控制释放过程。结果:bFGF可以通过静电作用与胶原基支架结合,均匀分布在多孔三维支架中,48h后,bFGF的溶出浓度趋于恒定,经过甲醛交联的支架对bFGF的结合量较高;bFGF的结合量受到壳聚糖的含量和交联方法的影响。结论:胶原基组织工程支架能够对生长因子在较长时间内实现控制释放,促进组织的再生修复。AIM:To control the release of the basic fibroblastic growth factor(bFGF) by co mbining bFGF with the collagen-based tissue-engineering scaffold so as to real ize tissue regeneration more effectively.METHODS:The ELISA method was used to analyze quantitatively the concentration of the bFGF released from the scaffold during the first 72 hours.RESULTS:bFGF could combine with the scaffold through a static reaction. The co mbination of the bFGF was effected by the composition of chitosan and the chemic al cross-linking method of the scaffold. CONCLUSION:The collagen tissue-engineering scaffold can control the release o f the growth factor in a long period, which will accelerate the rehabilitative p rocess of the tissue.

关 键 词：胶原基组织工程支架 成纤维生长因子 控制释放 组织再生 壳聚糖 

分 类 号：R318[医药卫生—生物医学工程]