低剂量γ射线诱导丝裂霉素C损伤小鼠胸腺细胞的适应性反应  

Adaptive Response of Murine Thymocytes Induced by Low Dose γ-rays before MMC Exposure

作　　者：孟庆勇[1] 高莉莉[1] 徐美奕[1] 

机构地区：[1]广东医学院分析中心同位素实验室,524023

出　　处：《天津医药》2004年第4期230-232,共3页Tianjin Medical Journal

基　　金：广东省高教厅自然科学重点科研项目(项目编号 :9603)

摘　　要：目的 :探讨低剂量辐射诱导小鼠拮抗丝裂霉素C(MitomycinC,MMC)损伤作用的机制。方法 :小鼠接受低剂量γ射线作用后6h ,再给予损伤剂量的MMC ,然后通过流式细胞术和 3H_TdR掺入法观察小鼠胸腺细胞周期和DNA含量的变化。结果 :50mGy和100mGy照射加MMC组小鼠胸腺细胞 3H_TdR自发掺入率、S期细胞百分率明显高于单纯MMC对照组 (P<0.01,P<0.05)。胸腺细胞G0/G1 期细胞百分率明显低于单纯MMC对照组 ,100mGy照射加MMC组小鼠胸腺细胞G2/M期细胞百分率明显低于单纯MMC对照组。结论 :低剂量γ射线对MMC损伤小鼠胸腺细胞自发掺入能力和细胞周期进程具有刺激作用。Objective:To observe the mechanism against MMC damage induced by low dose γ-rays on thymocytes in mice.Methods: The cycle progression and DNA contents of thymocytes were detected with flow cytometry and 3H-TdR 6 h after low dose radiation in male Kunming mice.Results: Compared with the simple use of MMC, The rates of spontaneous proliferation of thymocytes and S phase cell percentage significantly increased at the dose of 50 and 100 mGy combined with MMC(P<0.01, P<0.05).The percentage of G0/G1 phase cells was significantly decreased in irradiated and MMC group compared with that in simple MMC group.The percentage of G2/M phase cells was significantly reduced at dose of 100 mGy on thymocytes in irradiated and MMC group compared with that in simple MMC group.Conclusions: Low dose γ-rays effectively stimulates the synthesis of DNA and accelerates cell cycle progression on thymocytes in mice.

关 键 词：低剂量 Γ射线 诱导 丝裂霉素C 损伤小鼠 胸腺细胞 适应性反应 

分 类 号：X591[环境科学与工程—环境工程]