杂氮冠醚化Schiff碱钴(Ⅱ)配合物模拟磷酸二酯水解酶的研究  

The kinetic studies on the azo crown mono-schiff base Co(Ⅱ) complex as a mimic hydrolytic enzyme catalyzed BNPP hydrolysis

作　　者：胡伟[1] 刘富安[2] 谢家庆[1] 李建章[1] 秦圣英[1] 胡常伟[1] 曾宪诚[1] 

机构地区：[1]四川大学化学学院,四川成都610064 [2]四川理工学院化学系,四川自贡643000

出　　处：《化学研究与应用》2004年第2期215-218,共4页Chemical Research and Application

基　　金：国家自然科学基金资助项目 (No .2 0 173 0 3 8) ;四川省教育厅重点课题资助项目

摘　　要：含杂氮冠醚的Schiff碱过渡金属配合物作为模拟水解酶被用于催化BNPP水解 ,讨论了两种杂氮冠醚化单Schiff碱钴 (Ⅱ )配合物催化BNPP水解的动力学和机理 ,分析了反应体系的特征光谱变化。提出了配合物催化BNPP水解的动力学数学模型 ,结果表明 ,在反应过程中形成中间物种的假设是合理的 ;随着缓冲溶液pH的增大 ,两种配合物催化BNPP水解速率提高 ;两种配合物在催化BNPP水解中表现出好的催化活性。The transitional metal schiff base complexes containing azo crown as mimic hydrolytic enzyme were used for catalyzing BNPP hydrolysis. The kinetics and the mechanism on BNPP hydrolysis catalyzed by two azo crown mono-schiff base Co(Ⅱ) complexes were investigated. The change of characteristic spectra of the reactive systems was analyzed. A kinetic mathematical model of BNPP cleavage catalyzed by the azo crown mono-schiff base Co(Ⅱ) complexes was proposed. The results showed that the supposition of forming the intermediate in the reactive process was reasonable and the rate for the BNPP hydrolysis catalyzed by the complexes was increased following the increase of pH of the buffer solution. Two complexes exhibited high activity in the BNPP catalytic hydrolysis.

关 键 词：磷酸二酯水解酶 杂氮冠醚化西佛碱钴配合物 磷酸二酯键 BNPP水解 动力学 模拟酶 

分 类 号：Q556.1[生物学—生物化学]