血清饥饿法用于细胞周期同步化的方法学研究  

Methodological Study of Cell Cycle Synchronization by Serum Starvati on

作　　者：宋春娇[1] 吕冰洁[1] 张小玲[1] 史忠诚[1] 傅松滨[1] 李璞[1] 李钰[1] 

机构地区：[1]哈尔滨医科大学遗传学与细胞生物学教研室,黑龙江哈尔滨150086

出　　处：《中国地方病学杂志》2003年第4期362-364,共3页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金资助项目 ( 39970 39630 1 70 51 6 );黑龙江省自然科学基金资助项目( D980 9)

摘　　要：目的　探讨血清饥饿法进行细胞周期同步化实验的影响因素。方法　用无血清和低血清浓度培养基饥饿 Anip973和 AGZY83 -a两种人肺腺癌细胞系 ,分别在培养 48h、72 h和 5d时收集各组细胞 ,用流式细胞分析仪分析细胞周期各时相细胞百分数。结果　 Anip973细胞系在含 0 .2 % FBS的 RPMI 1640培养基中培养 5d得到理想结果 ,G0 -G1期细胞百分比高达 84.19%。 AGZY83 -a在含 0 .2 % FBS的 RPMI 1640培养基中培养 48h也获得较满意的结果 ,G0 -G1期细胞百分比达 61.3 0 %。结论　细胞类型、血清浓度和饥饿时间是血清饥饿法进行细胞周期同步化实验成功与否的影响因素。可应用血清饥饿法使不同细胞系同步于 G0Objective To determine the effective factors that involved in cell cycle synchronization by serum starvation.Methods By using serum-free and low concentra-tion se rum culture medium(CM),cell lines Anip973 and AGZY83-a,including were cultured ; and the cells were collected in 48 h,72 h and 5 days,respectively, then the results were analyzed by FCM(flow cytometry).Results Anip973 cells in RPMI 1640 CM containing 0.2% F BS Was cultured for 5 days,with the satisfactory result: 84.19% of Anip973 cells at G 0/G 1 phase. Furthermore, 61.30% of AGZY83-a cells at G 0/G 1 phase i n RPMI 1640 CM containing 0.2% FBS was gotten 48 hours later.Conclusions The influential factors of cell cycle synch ronization are cell characteristics, serum concentration and starvation period. Different cell lines may be synchronized in G 0-G 1 phase by serum starvatiom .

关 键 词：血清 饥饿法 细胞周期 方法学 流式细胞分析仪 

分 类 号：Q25[生物学—细胞生物学]