碱性成纤维细胞生长因子诱导非贴壁骨髓基质细胞向成骨细胞分化  

Effect of basic fibroblast growth factor on proliferation and differentiation of non-adherent stromal precursor cells

作　　者：邵君飞[1] 王旻晨[1] 保泽洛[1] 邢卫星[1] 季剑[1] 

机构地区：[1]苏州大学医学院解剖学教研室,苏州215007

出　　处：《解剖学杂志》2003年第3期226-229,共4页Chinese Journal of Anatomy

摘　　要：目的:探讨碱性成纤维细胞生长因子(bFGF)对非贴壁骨髓前体细胞(NASP)的增殖分化作用。方法:分离骨髓前体细胞体外培养,用碱性磷酸酶化学染色及图象分析测定细胞克隆数;采用免疫组化(ABC法)进行骨钙素及碱性磷酸酶(ALP)测定,研究bFGF对NASP的促增殖作用。结果:bFGF能促进NASP细胞增殖,诱导NASP细胞产生碱性磷酸酶及骨钙素。结论:bFGF主要作用于非贴壁生长的基质前体细胞,bFGF能增加骨的数量,促进骨样组织形成。Objective:To investigate the actions of basic fibroblast growth factor (bFGF) on the proliferation and differentiation of the non-adherent stromal precursor (NASP) cells. Methods:The osteogenic potential of the bone marrow stro-mal cells (BMSC) isolated from Wistar rats were cultured in the absence or presence of bFGF. After ALP cytochemistry performed, the colonies were counted by image analysis; The cells cultured in petri dishes were stained by the avidin-bi-otin-peroxidase complex (ABC) immunoperoxidase technique for double staining for osteocalcin. Results: bFGF not only stimulated the proliferation of NASP cells, but also enhanced the differentiation of NASP cells into osteoblasts. Conclusion: NASP cells are possible main target of anabolic action of bFGF and it may play an important role in fracture healing and bone formation.

关 键 词：碱性成纤维细胞生长因子 非贴壁骨髓基质细胞 成骨细胞 细胞分化 诱导 免疫组化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]