TGF-β1对Heymann肾炎肾小球细胞外基质的影响  

Effect of TGF-β1 on the extracellular matrix of glomeruli in the rats with Heymann nephritis

作　　者：罗伟[1] 王照明[2] 杨幼萍 张东生[4] 张慧敏[1] 张建民 

机构地区：[1]郑州市第三人民医院病理科,郑州450000 [2]浙江大学附属第一医院病理科,杭州310003 [3]浙江省温岭市第一人民医院病理科,温岭315700 [4]东南大学附属中大医院病理科,南京210009

出　　处：《临床与实验病理学杂志》2003年第5期539-542,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的　探讨TGF β1对不同病变阶段主动型Heymann肾炎肾小球细胞外基质的影响。 方法　建立主动型Heymann肾炎模型 ,在主动型Heymann肾炎第 4、6、8和 1 2周 ,用RT PCR法检测TGF β1mRNA在病变肾组织内的表达 ,并用免疫组化S P法检测纤维连接蛋白和Ⅳ型胶原在Heymann肾炎病变各阶段大鼠肾小球内的表达 ,再用计算机图像分析系统进行定量分析。结果　自主动型Heymann肾炎模型诱发后第 4周开始 ,TGF β1mRNA即有表达 ,至第 8周最明显 ,第 1 2周有所下调。主动型Heymann肾炎大鼠肾小球内纤维连接蛋白和Ⅳ型胶原的含量 ,随TGF β1表达的上调和病变的进展而增多。 结论　TGF β1可能通过刺激主动型Heymann肾炎大鼠肾小球上皮细胞产生过量细胞外基质 。Purpose To explore the effect of TGF β1 on the extracellular matrix of glomeruli in the rats with active Heymann nephritis(AHN) during the different periods. Methods After induction of AHN in SD rats, the expression of TGF β1 mRNA were measured with reverse transcription polymerase chain reaction(RT PCR) in the renal tissues of the rats with AHN at 4, 6, 8 and 12 weeks respectively. Meanwhile, the expression of fibronectin and type IV collagen in the glomeruli of the rats with AHN were studied with immunohistochemistry and computer imaging analysis during the above weeks. Results An up regulation of TGF β1 mRNA expression was observed in the renal tissues of the rats at 4 weeks after the induction of AHN. And this expression reached its peak at 8 weeks. Then a slight down regulation appeared in 12 weeks. The accumulation of fibronectin and type Ⅳ collagen were synchronously increased with the up regulation of TGF β1 mRNA expression and the lesion development of AHN. Conclusions TGF β1 may stimulate the glomerular epithelial cells to secret excessive amounts of extracellular matrix and mediate the development of AHN.

关 键 词：TGF-Β1 HEYMANN 肾炎 肾小球细胞 外基质 病变阶段 定量分析 

分 类 号：R692.31[医药卫生—泌尿科学]