Increase Renaturation Yield of Reteplase Using the Recombinant Human Protein Disulfide Isomerase  

作　　者：赵友春  Wang Ge  Kong Yang  WANG Yanbing  Zhang Changkai  Chen Chao  Liang Bufeng  

机构地区：[1]State Key Lab. of Microbial Technology, Shandong University, Jinan 250100, P.R. China Department of Science & Technology of Shandong Province, Jinan 250011, P.R.China [2]Shandong Younglong Biotechnology Co., Jinan 250100, P.R. China [3]State Key Lab. of Microbial Technology, Shandong University, Jinan 250100, P.R. China

出　　处：《High Technology Letters》2004年第1期49-51,共3页高技术通讯（英文版）

摘　　要：Reteplase, the recombinant type of novel tissue plasminogen activator (t-PA) variant, is a promising thrombolytics in clinics. Expressed in the form of an inclusion body, reteplase consists of about 40 % of the total intracellular proteins of Escherichia coli. The recombinant human protein disulfide isomerase (rhPDI) is used to increase the chance for the correct matching of the 18 hydrosulfide groups of the reteplase molecule in the renaturation process and it increase is the reteplase renaturation yield from 1%～2% to 15%～20% with a the purity aboue 99% and the specific activity of 5(105 IU/mg is reached. This novel method can reduce significantly the cost of production.

关 键 词：人体组织 血浆酶原催化剂 硫蛋白异构酶 重建 复活产量 心脑血管疾病 溶血栓剂 

分 类 号：R972[医药卫生—药品]