Preparation and Identification of Monoclonal Antibodies Against Vibrio anguillarum  

作　　者：陈师勇  Zhang Peijun  Mo Zhaolan  Zhang Zhendong  Zou Yuxia  Xu Yongli  

机构地区：[1]Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P.R.China Graduate School of Chinese Academy of Sciences, Beijing 100039, P.R.China [2]Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P.R.China

出　　处：《High Technology Letters》2004年第1期73-75,共3页高技术通讯（英文版）

基　　金：国家重点基础研究发展计划(973计划)

摘　　要：Monoclonal antibodies (Mabs) against V.anguillarum strain M3 are prepared, and their isotypes are also characterized. Among them, C1C5 is the only Mab which does not crossreact with other eleven non-V.anguillarum strains. The proteinase K digestion test shows that the epitopes recognized by C1C5, C6C3 and C6C32 Mabs contained protein. The periodate oxidation test showed that the epitopes recognized by Mabs except C1C5 are glycosylated. In addition, results of additivity test indicate that the epitopes recognized by C6C3 and C6C32 Mabs are similar, and quite different from those recognized by Mab C1C5.

关 键 词：单克隆抗体 水产业 抗原决定基 交叉反应 高碘酸盐氧化实验 鱼病 鳗弧菌 

分 类 号：S941.42[农业科学—水产养殖]