烟酰胺对大鼠胰岛β细胞的保护作用  

The protective effect of nicotinamide on pancreatic beta-cell in rats

作　　者：迟金华 辛颖[2] 王志超[2] 

机构地区：[1]辽宁省抚顺市第二医院儿科,113001 [2]中国医科大学第二临床学院儿科

出　　处：《中国糖尿病杂志》2004年第1期52-55,共4页Chinese Journal of Diabetes

摘　　要：目的　探讨烟酰胺 (NA)对糖尿病 (DM )大鼠胰岛 β细胞的保护作用。 　方法　DM组3 0只鼠 ,NA组 3 0只鼠 ,对照组 2 5只鼠。以NA(1g/kg)给大鼠灌胃 3d ,用链脲佐菌素 (STZ) (50mg/kg)一次性腹腔注射 ,观察血清一氧化氮 (NO)、丙二醛 (MDA)、胰腺匀浆一氧化氮合酶 (NOS)、超氧化物岐化酶 (SOD)及胰岛 β细胞凋亡的形态学改变。 　结果　 1d时NA组NO为 (69± 6) μmol/L ,DM组为 (10 7± 6) μmol/L ;5d时NA组血清MDA为 (5.9± 1.2 )nmol/L ,DM组为 (9.5± 1 0 )nmol/L ;5d时NA组胰腺匀浆SOD为 (14 7± 13 )nU /mg(pro) ,DM组为 (111± 6)nU/mg(pro) ,差异有显著意义 (P <0 .0 5) ;胰腺匀浆NOS三组差异无显著意义 (P >0 .0 5)。未见有胰岛 β细胞过度凋亡。　结论　NA可以清除STZ产生的NO及氧自由基 ,保护胰岛 β细胞 。ObjectiveTo clarify the protective effect of n ic tinamide on beta-cell of islet Langerhands in rats.Methods1 15 rats were divided into control (A group), diabetes (B group) and NA (C group) . NA group rats ingested NA every day. After three days, 2% streptozotocin was i njected intraperitoneum once. Tests were madefor nitric oxide (NO), matondialdeh yde (MDA) in serum, and nitric oxide synthase (NOS), superoxide dismutase (SOD) in pancreas. Apoptotic morphology of Langerhands was checked.Results On day 1, the level of NO was 69.0±5.7 μmol/L in NA group, 106.5±6.57 μmol/L in diabetes group. On day 5, MDA was 5.88±1.21 nmol/L in NA group, 9.5 1±0.97 nmol/L in diabetes group. On day 5, SOD was 146.9±13.1 nU/mg(pro) in NA group, 111.3±6 0 nU/mg(pro) in diabetes group. The difference was significant (P<0.05). There was no difference in NOS among three groups (P>0.05). A nd there were morphological evidences for apoptosis of beta-cells in B group. The apoptosis of beta-cells in C and A groups were not founded.Conclu sionNA acting as a scavenger may protect beta-cells of Langerhands fr om apoptosis. NA may protect rats from sufferring diabetes induced by streptozot ocin.

关 键 词：烟酰胺 大鼠 胰岛Β细胞 NA 一氧化氮 T1DM 胰岛素 

分 类 号：R587.1[医药卫生—内分泌]