三种鼠尾草注射液对离体大鼠心肌缺血再灌注损伤的保护作用  

Effects of Salvia Yunnanensis C.H.Wright,Salvia Przerwalskii Maxim, Salvia Przewalskii Maxim Var Mandarinorum Stib on Acute Myocardial Ischemia and Reperfusion Injury in Rats

作　　者：张荣平[1] 李惠兰[2] 郑春兰[2] 秦剑[2] 

机构地区：[1]昆明医学院药学院植物化学教研室,昆明650031 [2]昆明医学院药理教研室,昆明650031

出　　处：《中国血液流变学杂志》2004年第1期18-21,共4页Chinese Journal of Hemorheology

基　　金：云南省应用基础研究基金资助 ( 98C0 67M)

摘　　要：目的　研究云南产鼠尾草属药物滇丹参、甘西鼠尾、褐毛甘西鼠尾对心肌缺血再灌注损伤的保护作用 ,并与丹参进行对比。方法　采用Langendorff离体心脏灌流法 ,全心停灌后再灌流造成心肌缺血再灌注模型 ,通过动脉套管侧管给药。测定再灌注 30min后 ,灌流液中CK、LDH、SOD、GSH Px和MDA含量 ;同时测定离体心脏冠脉流量、心肌收缩幅度和心率。结果　滇丹参、甘西鼠尾、褐毛甘西鼠尾可以对抗离体大鼠心脏停灌后再灌注引起的冠脉流量、心肌收缩幅度和心率的降低 (P <0 .0 1,P <0 .0 5 ) ;降低灌流液中CK、LDH和MDA含量 ,提高SOD和GSH Px的活力 (P <0 .0 1,P <0 .0 5 )。结论　滇丹参、甘西鼠尾、褐毛甘西鼠尾对在体和离体缺血 /再灌注心肌有保护作用。Objective To observe the effects of Salvia yunnanensis C.H.Wright,Salvia przerwalskii Maxim and Salvia przewalsk Maxim var mandarinorum stib in myocardial ischemia/ reperfusion in rats.Methods Via the Langendorff heart reperfused model,5 mintutes after using the drugs,we have made the rat's heart ischemia for 15 minutes and reperfusion for 30 minutes.During the whole process,we examined the flow of coronary artery,degree of myocardial contraction,heart rate and the concentration of the CK,LDH,SOD,GSH-Px and MDA in perfusion liquid.Results In the model of Langendorff heart perfused,the flow of coronry artery,degree of myocardial contraction,heart rate of the treatment groups are obviously higher compared with the untreated group(P<0.01,P<0.05).The concentration of the CK,LDH and MDA decreased and that of SOD and MDA rised (P<0.01,P<0.05).Conclusion Salvia yunnanensis C.H.Wright,Salvia przerwalskii Maxim,Salvia przewalskii Maxim var mandarinorum stib have the cardioprotective effects in myocardial ischemia/reperfusion in rat hearts.

关 键 词：鼠尾草注射液 离体大鼠 心肌缺血 再灌注损伤 保护作用 血流动力学 

分 类 号：R542.2[医药卫生—心血管疾病] R285.5[医药卫生—内科学]