关于N-乙酰氨基半乳糖转移酶基因家族cDNA扩增的初步研究  

Primary Study of N-acetylgalactosaminyltransferases Gene Family cDNA Amplification

作　　者：贾伟[1] 吴士良[1] 

机构地区：[1]苏州大学基础医学系生物化学与分子生物学教研室,苏州215007

出　　处：《中国血液流变学杂志》2004年第1期48-49,56,共3页Chinese Journal of Hemorheology

摘　　要：目的　通过实验方法扩增N 乙酰氨基半乳糖转移酶基因家族cDNA。方法　主要通过PCR技术 ,用各种N 乙酰氨基半乳糖转移酶基因家族cDNA的特异性引物进行扩增并结合电泳技术 ,用不同的限制性内切酶进行酶切鉴定。结果　通过电泳图谱和酶切图谱初步证实了这一家族的不同成员均已得到有效的扩增。结论　成功扩增了N 乙酰氨基半乳糖转移酶基因家族cDNA ,并为其文库的建立打下良好的基础。Objective To amplify N-acetylgalactosaminyltransferases gene family cDNA by experimental methods.Methods Mainly by means of PCR, various specific primers were used for amplification of N-acetylgalactosaminyltransferases gene family cDNA. Meanwhile, electrophoresis technique was combined for identification of N-acetylgalactosaminyltransferases gene family.Results Through patterns of electrophoresis and restriction endonuclease, it was rudimentarily proved that N-acetylgalactosaminyltransferases gene family cDNA had been successfully amplified. Conclusion Successful amplification of N-acetylgalactosaminyltransferases may be used as a solid foundation of making N-acetylgalactosaminyltransferases gene family cDNA library.

关 键 词：N-乙酰氨基半乳糖转移酶基因家族 cDNA扩增 PCR 限制性内切酶 

分 类 号：Q555.4[生物学—生物化学]