镧与邻菲咯啉和联吡啶的混配物的合成及其与DNA作用的研究  

Synthesis of Mixed Complexes of Lanthanum with 2,2-Bipyridyl and 1,10-Phenanthroline and Suudy of the Interaction of the Complexes with DNA

作　　者：方光荣[1] 李玲[1] 蔡朝霞[1] 刘立明[1] 宋功武[1] 

机构地区：[1]湖北大学化学与材料科学学院,湖北武汉430062

出　　处：《光谱学与光谱分析》2004年第3期327-329,共3页Spectroscopy and Spectral Analysis

基　　金：中国分析测试基金 (99 1 1 )资助项目

摘　　要：合成了稀土镧 (La)与 2 ,2 联吡啶 (dpy) ,1,10 邻菲咯啉 ( phen)的混配物 ,并进行了红外和热分析的表征 ,证实了其混配物的结构为La(dpy) ( phen)Cl3 。并对其与DNA作用的紫外 可见光谱和共振光散射光谱作了初步的研究。研究结果表明 ,混配物的吸收峰随着DNA的加入而发生减色效应 ,而DNA浓度继续增高 ,吸收峰又增高 ,说明此混配物与DNA先是嵌入结合 ,而后伴随有静电结合。而此混配物在 4 70nm处的共振光散射峰随着DNA的加入增强 ,则表明混配物在DNA分子表面进行长距离自组装。研究证明所合成的配合物能够与DNA作用 。A mixed complex of La with 2,2-bipyridyl (dpy) and 1,10-phenanthroline(phen) was synthesized. Elemental analysis, IR and TGA of the complex were investigated, thus it was proved that the conformation of it was La(dpy) (phen) Cl-3. The interaction of the complex with DNA has been studied also. The spectra showed that the interaction between La(dpy) (phen) Cl-3 and DNA experienced intercalation of long-range self-assembly. The study made it clear that the synthesized mixed complex La(dpy) (phen)Cl-3 can interact with DNA, and La(dpy) (phen) Cl-3 can be used for probing a nucleic acid.

关 键 词：镧 邻菲咯啉 联吡啶 混配物 合成 DNA 红外光谱 可见光谱 共振光散射光谱 核酸探针 

分 类 号：Q523[生物学—生物化学] O614.331[理学—无机化学]