桑色素合镧(Ⅲ)与核酸作用的荧光光谱及其分析应用  

Study on the Fluorescence Spectra of LaL_3 with DNA

作　　者：王兴民[1] 宋玉民[2] 滕秀兰[2] 耿志远[2] 杨培菊[2] 杨美玲[2] 

机构地区：[1]甘肃农业大学基础科学系,甘肃兰州730070 [2]西北师范大学化学化工学院,甘肃兰州730070

出　　处：《光谱学与光谱分析》2004年第3期339-341,共3页Spectroscopy and Spectral Analysis

基　　金：甘肃省自然科学基金 (ZS0 2 1 A2 5 0 0 5 Z);西北师范大学基金(KJCXGC 0 2 0 9)资助

摘　　要：采用荧光法研究了桑色素合镧 (Ⅲ )配合物与核酸的作用。研究了溶液的pH值、缓冲溶液含量、有机试剂、外加干扰离子对桑色素合镧 (Ⅲ )配合物与核酸体系荧光光谱的影响 ,同时还研究了变性DNA与桑色素合镧 (Ⅲ )配合物之间的作用。在溶液 pH =8 0～ 9 0的条件下 ,体系的荧光强度最大 ,最大激发波长为387nm ,最大发射波长为 5 35nm。缓冲溶液的用量控制在 10 % (体积 ) ,乙醇用量也控制在 10 % (体积 )。配合物荧光强度在核酸存在时有所增加 ,据此确定了测定核酸的一种方法 ,简便、快速 ,线性范围宽 ,灵敏度较高 ,结果准确。并对可能的增色效应和酸度对荧光光谱影响的原因进行了讨论。A fluorescence method is presented for the determination of DNA. The method is based on the interaction of LaL3 [L = morin (2 3, 4 5, 7-Pentahydroxyflavone), 2'-OH group deprotonated] with DNA in NH3.H2O-NH4Cl buffer of pH = 8.0, with lambda(ex) at 387 nm, and lambda(em) at 535 nm. Enhanced fluorescence was observed for LaL3 in the presence of DNA and in the presence of buffer solution of 10% (phi) and ethanol of 10% (phi). The linear range of determination was between 0 and 15 mug.mL(-1) for DNA. The method proved to be simple, easy and sensitive for the determination of DNA. The mechanism of enhancing and the reasons for the effects of acid were also discussed.

关 键 词：桑色素合镧 核酸 荧光光谱 DNA 抗肿瘤活性 

分 类 号：Q523[生物学—生物化学] R979.1[医药卫生—药品]