Construct Hairpin RNA to Fight Against Rice Dwarf Virus  

作　　者：MAZhong-Liang YANGHuai-Yi WANGRong TIENPo 

机构地区：[1]DepartmentofMolecularVirologyandBioengineering,Instituteofmicrobiology,TheChineseAcademyofSciences,Beijing100080,China

出　　处：《Acta Botanica Sinica》2004年第3期332-336,共5页Acta Botanica Sinica（植物学报：英文版）

摘　　要：Hairpin RNA (hpRNA) can induce post-transcriptional gene silencing, and was constructed with128-754 bp of segment 8 of rice dwarf virus then placed under the control of the CaMV35S promoter andused to transform rice cultivar "Zhonghua 11" via Agrobacterium tumefaciens. A total of 12 independentlines containing hpRNA were obtained as demonstrated by Southern blotting analysis. Challenge inocula-tion with rice dwarf virus (RDV) viruliferous leafhoppers (Nephotettix cincticeps) showed that T1 plantscontaining the hpRNA transgene displayed high resistance or delayed and attenuated viral symptoms. Incontrast, transgenic lines expressing sense RNA showed severe symptoms similar to control plants trans-formed with the vector alone. These results suggest that hpRNA confers high resistance to RDV intransgenic rice.

关 键 词：转录 基因沉默 水稻矮缩病毒 转基因水稻 发夹结构 双链RNA 

分 类 号：S511.034[农业科学—作物学] Q943[生物学—植物学]